Erythritol is an important sweetener ingredient and chemical precursor for synthesizing materials with phase transition behavior. Commercial erythritol is primarily produced by industrial fermentation. Further strain engineering necessitates the development of high throughput screening method for rapid detection and screening of mutant strain libraries. In this work, we took advantage of the erythritol-responsive transcription factor EryD, and constructed a sensor-regulator system for rapid screening and characterization of erythritol overproducers. We configured the optimal architecture of the EryD sensor-regulator construct with improved sensitivity, specificity and dynamic response range. Coupled with mutagenesis and strain screening based on biosensors, we rapidly screened and characterized a strain library containing 1152 mutants derived from combined UV and ARTP mutagenesis, in a relatively short period of time (1 week). The optimal strain produced more than 148 g/L erythritol in bench-top reactors. This work provides a reference for other metabolic engineering researchers to develop industrially-relevant strains. The reported framework enables us to rapidly improve strain performance and engineer efficient microbial cell factories for industrial applications.

赤藓糖醇是重要的甜味剂成分和化学前体，用于合成具有相变行为的材料。商业赤藓糖醇主要通过工业发酵生产。进一步的菌株工程需要开发用于快速检测和筛选突变菌株文库的高通量筛选方法。在这项工作中，我们利用了赤藓糖醇反应性转录因子EryD，并构建了一个传感器-调节器系统，用于快速筛选和表征赤藓糖醇过量生产者。我们配置了具有改进的灵敏度，特异性和动态响应范围的EryD传感器-调节器构建体的最佳架构。结合基于生物传感器的诱变和菌株筛选，我们在相对较短的时间内（1周）快速筛选并鉴定了包含1152个源自UV和ARTP联合诱变的突变体的菌株文库。最佳菌株在台式反应器中产生了超过148 g / L的赤藓糖醇。这项工作为其他代谢工程研究人员开发与工业相关的菌株提供了参考。报告的框架使我们能够快速提高菌株性能，并为工业应用设计高效的微生物细胞工厂。