Abstract To develop a potential bio herbicide, herbicide activity and crop safety of fungal strain HZ-31 isolated from naturally infected weed Cirsium arvense (L.) Scop. were investigated. The study was conducted in vitro and in vivo during the summers of 2017 and 2018. Strain HZ-31 showed broad-spectrum herbicidal activity, both in vitro and in vivo, when targeting Elsholtzia densa Benth., Polygonum lapathifolium L., Lepyrodiclis holosteoides (C.A. Mey.) Fenzl ex Fisch. & C.A. Mey., Avena fatua L., Chenopodium album L. and Polygonum aviculare L. Exposure to HZ-31 in vitro induced heavy levels of damage in the leaves of E. densa, A. fatua, and P. aviculare. Exposure to HZ-31 in vivo resulted in high mortality in E. densa, A. fatua, and P. lapathifolium. Although the exposure of various weeds to HZ-31 caused severe damage, the strain did not cause any deleterious effects in Vicia faba L. and Pisum sativum L., two of the five crops tested. Our results suggest that fungal strain HZ-31 could be a potential mycoherbicide candidate for weed control in V. faba and P. sativum fields. Based on its morphological characteristics and ITS nucleotide sequence analysis, the fungus was classified as Trichoderma polysporum (Louk:Fr.) Rifai. New perspectives for weeds control by T. polysporum and its use in V. faba and P. sativum crops were prospected, considering the bioherbicide selectivity described in this study.

中文翻译：

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使用多孢木霉菌株 HZ-31 进行生物杂草控制

摘要 为了开发一种潜在的生物除草剂、除草剂活性和作物安全的真菌菌株 HZ-31，从自然感染的杂草 Cirsium arvense (L.) Scop 中分离出来。被调查。该研究是在 2017 年和 2018 年夏季进行的体外和体内研究。当靶向 Elsholtzia densa Benth.、Polygonum lapathifolium L.、Lepyrodiclis holosteoides 时，菌株 HZ-31 在体外和体内均显示出广谱除草活性（ CA Mey。）Fenzl ex Fisch。& CA Mey.、Avena fatua L.、Chenopodium album L. 和 Polygonum aviculare L. 在体外暴露于 HZ-31 会导致 E. densa、A. fatua 和 P. aviculare 的叶子严重受损。体内暴露于 HZ-31 导致 E. densa、A. fatua 和 P. lapathifolium 的高死亡率。尽管各种杂草暴露于 HZ-31 造成了严重损害，该菌株不会对蚕豆和豌豆（测试的五种作物中的两种）造成任何有害影响。我们的结果表明，真菌菌株 HZ-31 可能是一种潜在的真菌除草剂候选物，用于控制蚕豆和苜蓿田的杂草。根据其形态特征和 ITS 核苷酸序列分析，该真菌被归类为多孢木霉 (Louk:Fr.) Rifai。考虑到本研究中描述的生物除草剂选择性，展望了多孢霉控制杂草的新前景及其在蚕豆和苜蓿作物中的应用。根据其形态特征和 ITS 核苷酸序列分析，该真菌被归类为多孢木霉 (Louk:Fr.) Rifai。考虑到本研究中描述的生物除草剂选择性，展望了多孢霉控制杂草的新前景及其在蚕豆和苜蓿作物中的应用。根据其形态特征和 ITS 核苷酸序列分析，该真菌被归类为多孢木霉 (Louk:Fr.) Rifai。考虑到本研究中描述的生物除草剂选择性，展望了多孢霉控制杂草的新前景及其在蚕豆和苜蓿作物中的应用。