Sensitive and accurate detection methods for infectious viruses are the pressing need for effective disease diagnosis and treatment. Herein, based on V₂O₅ nanoparticles-encapsulated liposomes (VONP-LPs) we demonstrate a dual-modality sensing platform for ultrasensitive detection of the virus. The sensing performance relies on intrinsic peroxidase and electrochemical redox property of V₂O₅ nanoparticles (V₂O₅ NPs). The target-specific antibody-conjugated VONP-LPs and magnetic nanoparticles (MNPs) enrich the virus by magnetic separation and the separated VONP-LPs bound viruses are hydrolyzed to release the encapsulated V₂O₅ NPs. These released nanoparticles from captured liposomes act as peroxidase mimics and electrochemical redox indicator resulting in noticeable colorimetric and robust electrochemical dual-signal. Utilizing the superiority of dual-modality sensor with two quantitative analysis forms, norovirus like particles (NoV-LPs) can be detected by electrochemical signals with a wide linear range and low detection limit. To verify the applicability in real samples, norovirus (NoV) collected from actual clinical samples are effectively-identified with excellent accuracy. This proposed detection method can be a promising next-generation bioassay platform for early-stage diagnosis of virus disease and surveillance for public health.

敏感而准确的传染病检测方法是有效诊断和治疗疾病的迫切需求。本文中，基于V ₂ O ₅纳米颗粒包裹的脂质体（VONP-LP），我们展示了用于病毒超灵敏检测的双模式传感平台。传感性能取决于V ₂ O ₅纳米颗粒（V ₂ O ₅ NP）的固有过氧化物酶和电化学氧化还原特性。结合有靶标特异性抗体的VONP-LP和磁性纳米颗粒（MNP）通过磁性分离富集病毒，并且分离的VONP-LPs结合病毒被水解以释放包封的V ₂ O ₅NP。这些从捕获的脂质体释放的纳米粒子充当过氧化物酶模拟物和电化学氧化还原指示剂，从而产生明显的比色和稳健的电化学双信号。利用具有两种定量分析形式的双模态传感器的优势，可通过线性范围宽且检测限低的电化学信号检测诺如病毒颗粒（NoV-LPs）。为了验证在真实样品中的适用性，从实际临床样品中收集的诺如病毒（NoV）得到了有效鉴定，准确性很高。提出的检测方法可以成为有希望的下一代生物测定平台，用于病毒疾病的早期诊断和公共卫生监测。