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Mechanism Analysis of a Novel Angiotensin-I-Converting Enzyme Inhibitory Peptide from Isochrysis zhanjiangensis Microalgae for Suppressing Vascular Injury in Human Umbilical Vein Endothelial Cells
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2020-04-02 , DOI: 10.1021/acs.jafc.0c00925
Jiali Chen 1 , Li Tan 1 , Chengyong Li 1, 2, 3 , Chunxia Zhou 1, 2, 3 , Pengzhi Hong 1, 2 , Shengli Sun 1 , Zhong-Ji Qian 1, 2, 3
Affiliation  

Microalgae are primary producers with multiple nutrients in aquatic environments and mostly have applications in biological feed and fuel industry. There are few studies assessing the angiotensin-I-converting enzyme (ACE) inhibition potential of Isochrysis zhanjiangensis, other than its antioxidant potential. In this study, we evaluated a peptide from I. zhanjiangensis (PIZ, FEIHCC) and its vascular endothelial factors and mechanism in human umbilical vein endothelial cells (HUVEC). The results reveal that PIZ (IC50 = 61.38 μM) acts against ACE in a non-competitive binding mode. In addition, PIZ inhibits angiotensin II (Ang II)-induced vascular factor secretion and expression by blocking inflammation and apoptosis through nuclear factor κB (NF-κB), nuclear erythroid 2-related factor 2 (Nrf2), mitogen-activated protein kinases (MAPKs), and the serine/threonine kinase (Akt) signal pathways. This study reveals that PIZ has potential to be developed as a therapeutic agent for hypertension and provides a new method of high-value utilization of I. zhanjiangensis.

中文翻译:

湛江等鞭藻微藻新的血管紧张素转化酶抑制肽抑制人脐静脉内皮细胞血管损伤的机制分析

微藻是在水生环境中具有多种营养成分的主要生产者,并且主要在生物饲料和燃料工业中应用。除抗氧化潜力外,很少有研究评估湛江鞭毛虫的血管紧张素转换酶(ACE)抑制潜力。在这项研究中,我们评估了湛江一线虫肽(PIZ,FEIHCC)及其在人脐静脉内皮细胞(HUVEC)中的血管内皮因子和机制。结果显示PIZ(IC 50= 61.38μM)以非竞争性结合模式对抗ACE。此外,PIZ通过阻断核因子κB(NF-κB),核类红细胞2相关因子2(Nrf2),促分裂原活化蛋白激酶(NF-κB)的炎症和凋亡来抑制血管紧张素II(Ang II)诱导的血管因子的分泌和表达( MAPKs)和丝氨酸/苏氨酸激酶(Akt)信号通路。这项研究表明,PIZ有潜力被开发为高血压的治疗剂,并为湛江一带的高价值利用提供了一种新的方法。
更新日期:2020-04-03
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