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Lysosome-Targeting Iridium(III) Probe with Near-Infrared Emission for the Visualization of NO/O2•- Crosstalk via In Vivo Peroxynitrite Imaging.
Analytical Chemistry ( IF 7.4 ) Pub Date : 2020-04-01 , DOI: 10.1021/acs.analchem.0c00259
Weijun Wu 1 , Cheng Zhang 1 , Thomas W Rees 1 , Xinxing Liao 1 , Xu Yan 1 , Yu Chen 1 , Liangnian Ji 1 , Hui Chao 1, 2
Affiliation  

Nitric oxide (NO) and superoxide anions (O2•-) are two noteworthy reactive species implicated in various physiological and pathological processes, such as ROS-induced lysosomal cell death. The interaction ("crosstalk") between them may form a new mediator peroxynitrite (ONOO-) which has implications for cancer, diabetes, Alzheimer's disease, and liver-damage. It is therefore essential to investigate lysosomal NO/O2•- crosstalk in vivo through ONOO--responsive molecular tools in order to fully comprehend the physiological and pathological mechanisms involved. In this study, a lysosome-targeting iridium(III) complex, Ir-NIR, has been investigated as a near-infrared (NIR) phosphorescent probe for visualizing NO/O2•- crosstalk by the phosphorescent detection of endogenous ONOO- levels in vivo. Ir-NIR exhibits a rapid (within 200 s), highly sensitive, and approximately 100-fold enhanced response to ONOO- in phosphorescence intensity. Thus, these characteristics, coupled with good cell permeability and low cytotoxicity, enable the probe to be used to detect intracellular ONOO- living organisms both in vitro and in vivo.

中文翻译:

具有近红外发射特性的靶向溶酶体的铱(III)探针,可通过体内过氧亚硝酸盐成像可视化NO / O2•-串扰。

一氧化氮(NO)和超氧阴离子(O2•-)是两个值得注意的反应性物种,与各种生理和病理过程有关,例如ROS诱导的溶酶体细胞死亡。它们之间的相互作用(“串扰”)可能形成新的过氧亚硝酸盐(ONOO-),对癌症,糖尿病,阿尔茨海默氏病和肝损伤都有影响。因此,有必要通过ONOO反应分子工具研究体内溶酶体NO / O2•-串扰,以充分理解所涉及的生理和病理机制。在这项研究中,已研究了靶向溶酶体的铱(III)络合物Ir-NIR作为近红外(NIR)磷光探针,用于通过磷光检测体内内源性ONOO-水平来可视化NO / O2•-串扰。 。Ir-NIR表现出快速(200秒以内),高度敏感,并且对磷光强度的ONOO-响应增强了约100倍。因此,这些特性,加上良好的细胞渗透性和低细胞毒性,使得该探针可用于在体内和体外检测细胞内ONOO活生物体。
更新日期:2020-04-23
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