Inorganic nanomaterials have gathered significant attention due to their biological enzyme-like catalytic activities, which are currently being utilized to display excellent biomedical applications. Among these, inorganic nanomaterials exhibiting catalase enzyme-like properties are of particular interest because they can impart protection to mammalian cells from cellular hydrogen peroxide (H₂O₂) and other free radicals. In this study, we have investigated the utility of bovine serum albumin (BSA) coated magnesium nanoparticles (BSA-MgNPs) with respect to the protection of mammalian hepatic cells with depleted cellular catalase enzyme. We observed that BSA-MgNPs lead to the rapid breakdown of H₂O₂ and therefore protect mammalian cells exposed to 3-amino-1,2,4-triazole (3-AT), an irreversible catalase enzyme inhibitor. Results revealed that 3-AT induces oxidative stress by challenging the glutathione (GSH) and catalase enzyme, thus altering the cellular redox balance, which leads to the formation of reactive oxygen species (ROS) in human hepatocytes (WRL-68). Results showed that BSA-MgNPs impart protection to the catalase depleted hepatic cells. Expression analysis showed that BSA-MgNPs favorably modulate cellular antioxidant proteins/enzymes and genes such as catalase, thioredoxin reductase (TrxR), peroxiredoxin 6 (Prx6), and Hsp 70 (heat shock protein). These results conclude that BSA-MgNPs impart protection to WRL-68 cells from acatalasia to display better cell survival as well as a remarkable decrease in the intracellular ROS levels.

中文翻译：

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BSA装饰的镁纳米粒子可清除人肝细胞中的过氧化氢

无机纳米材料由于其类似于生物酶的催化活性而备受关注，目前已被用于显示出色的生物医学应用。其中，表现出过氧化氢酶样性质的无机纳米材料特别令人关注，因为它们可以保护哺乳动物细胞免受细胞过氧化氢（H ₂ O ₂）和其他自由基的侵害。在这项研究中，我们已经研究了牛血清白蛋白（BSA）包被的镁纳米颗粒（BSA-MgNPs）在用耗尽的细胞过氧化氢酶保护哺乳动物肝细胞方面的作用。我们观察到BSA-MgNPs导致H ₂ O ₂迅速分解因此可以保护暴露于3-氨基-1,2,4-三唑（3-AT）（一种不可逆的过氧化氢酶抑制剂）的哺乳动物细胞。结果显示3-AT通过挑战谷胱甘肽（GSH）和过氧化氢酶来诱导氧化应激，从而改变细胞氧化还原平衡，从而导致人肝细胞中形成活性氧（ROS）（WRL-68）。结果表明，BSA-MgNPs对过氧化氢酶耗竭的肝细胞具有保护作用。表达分析表明，BSA-MgNPs可以很好地调节细胞抗氧化剂蛋白/酶和基因，例如过氧化氢酶，硫氧还蛋白还原酶（TrxR），过氧化物酶6（Prx6）和Hsp 70（热激蛋白）。这些结果得出结论，BSA-MgNPs赋予了来自脱氢液的WRL-68细胞保护作用，以显示更好的细胞存活率以及细胞内ROS水平的显着降低。