The peroxynitrite ion (ONOO⁻) has important roles in many biological processes. We have developed a multicolor ONOO⁻‐sensing probe (SiONNOH) that undergoes deprotonation and desilylation processes, which result in several changes in the emission wavelengths. In response to different concentrations of ONOO⁻, the probe exhibits fluorescence changes from pink (595 nm at 2 eq. ONOO⁻) to green (540 nm at 6 eq. ONOO⁻) via orange (3 eq. ONOO⁻) and yellow (4 eq. ONOO⁻) under physiological conditions until no fluorescence signal is observed after ONOO⁻ is completely eliminated by lipoic acid. The probe shows the high selectivity for ONOO⁻ and the limit of detection is calculated to be 1.27 μM. Moreover, the probe shows the capacity to monitor the concentration ranges of ONOO⁻ through multicolor fluorescence in living cells, which will greatly facilitate the rapid detection of ONOO⁻ concentration ranges by the naked eye under a UV light without any precision instrumentation.

中文翻译：

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基于甲硅烷基醚的荧光探针可通过多色发射快速监测活细胞中内源性过氧亚硝酸盐的浓度和成像。

过亚硝酸盐离子（ONOO ^- ）在许多生物过程的重要作用。我们已经开发出一种多色ONOO ^- -sensing探头（SiONNOH）经历去质子化和脱硅基化过程，这导致在发射波长的一些变化。响应于不同浓度ONOO的^- ，探针显示荧光从粉红色改变（595纳米在2当量ONOO。^- ）到绿色（540纳米在6当量ONOO。^-通过橙色）（ONOO 3当量^- ）和黄色（ 4当量ONOO。^-生理条件，直到没有荧光信号ONOO后观察下）^-是完全由硫辛酸消除。探针显示出对ONOO的高选择性^-并且计算出的检测下限为1.27μM。此外，探针显示监视ONOO的浓度范围的能力^-通过多色荧光活细胞中，这将大大促进ONOO的快速检测^-的浓度范围由在UV光下肉眼没有任何精密仪器。