In a bioelectrochemical system (BES), microbial community of anode biofilm is crucial to BES performance. In this study, the stratified pattern of community structure and activity of an anode‐respiring biofilm in a BES fueled with brewery wastewater was investigated over time. The anode biofilm exhibited a superior performance in the removal of ethanol to that of an open‐circuit system. The electrical current density reached a high level of 0.55mA/cm² with a Coulombic efficiency of 71.4%, but decreased to 0.18mA/cm² in the late stage of operation. A mature biofilm developed a more active outer layer covering a less active inner core, although the activities of the outer and inner layers of biofilm were similar in the early stage. More Geobacter spp., typical exoelectrogens, were enriched in the outer layer than in the inner layer of biofilm in the early stage, while more Geobacter spp. were distributed in the inner layer than in the outer layer in the late stage. The inactive and Geobacter ‐occupied inner layer of biofilm might be responsible for the decreased electricity generation from wastewater in the late stage of operation. This study provides better understanding of the effect of anode biofilm structure on BES performance.

中文翻译：

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电化学辅助啤酒废水处理过程中阳极生物膜内的分层微生物结构和活性。

在生物电化学系统 (BES) 中，阳极生物膜的微生物群落对 BES 性能至关重要。在这项研究中，随着时间的推移，研究了以啤酒废水为燃料的 BES 中阳极呼吸生物膜的群落结构和活性的分层模式。阳极生物膜在乙醇去除方面表现出优于开路系统的性能。电流密度达到0.55毫安/ cm的高级别²用的71.4％的库仑效率，但是降低到0.18毫安/厘米²在操作的后期阶段。成熟的生物膜形成了一个更活跃的外层，覆盖了一个不太活跃的内核，尽管生物膜外层和内层的活动在早期是相似的。更多地杆菌spp. 是典型的外生电原，早期在生物膜外层比内层富集，而Geobacter spp. 后期分布在内层多于外层。生物膜内层不活跃且被地杆菌占据的生物膜可能是运行后期废水发电量减少的原因。这项研究为阳极生物膜结构对 BES 性能的影响提供了更好的理解。