Cerium dioxide nanoparticles (CeO₂NPs) with a high value of ζ‐potential (≥30 mV) have been synthesized in reverse microemulsions and they are able to form the high‐stable aqueous suspension without any additional stabilizers. It has been shown that the interaction of such CeO₂ NPs with transport proteins, such as BSA, affects their molecular conformation and biochemical activity. The observed changes in the UV‐absorbance spectrum and intrinsic fluorescence quenching of BSA molecule are indicative of the occurrence of structural changes caused by binding with the surface of CeO₂NPs. Low affinity between BSA and CeO₂ NPs has been confirmed by differential scanning calorimetry (DSC). Moreover, CeO₂NPs can act as regenerative free‐radical scavengers, and their antioxidant activity depends on the concentration. The positive charge of CeO₂NPs can be attributed to their low toxicity toward human malignant lymphocytes MT‐4 and breast cancer cells MCF‐7 however, the morphofunctional features of MCF‐7 cells interacting with CeO₂NPs are indicative of the decrease in oncogenicity.

中文翻译：

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二氧化铈纳米粒子的生物活性。

已经在反相微乳液中合成了具有高 ζ 电位值（≥30 mV）的二氧化铈纳米粒子（CeO ₂ NPs），它们能够在没有任何额外稳定剂的情况下形成高稳定性的水悬浮液。已经表明，此类 CeO ₂ NPs 与转运蛋白（如 BSA）的相互作用会影响其分子构象和生化活性。观察到的 BSA 分子的紫外吸收光谱和固有荧光猝灭的变化表明发生了由与 CeO ₂ NPs表面结合引起的结构变化。BSA 和 CeO ₂ NPs之间的低亲和力已通过差示扫描量热法 (DSC) 得到证实。此外，CeO ₂NPs可以作为再生自由基清除剂，其抗氧化活性取决于浓度。CeO ₂ NPs的正电荷可归因于它们对人恶性淋巴细胞 MT-4 和乳腺癌细胞 MCF-7 的低毒性，然而，MCF-7 细胞与 CeO ₂ NPs相互作用的形态功能特征表明致癌性降低.