Droplet microfluidic assays are rapidly gaining popularity because they enable analysis of biochemical reactions, individual cells or small cell populations with high sensitivity, precision and accuracy in a high-throughput manner. Nonetheless, there is a demand for user-friendly and low-cost droplet analysis technology. In this article, we meet this demand by developing two droplet analysis pipelines via free open-source software CellProfiler (CP) and its companion (CellProfiler Analyst, CPA). To illustrate the competence of the pipelines as an independent, and freely accessible droplet analysis tool for any researcher without the need of programming skills, we show (i) droplet digital quantification of viable fluorescent bacteria using single-color images and (ii) analysis of a multi-color fluorescence image with droplets containing different chemical compositions.

中文翻译：

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使用用户友好的免费软件CellProfiler进行液滴图像分析

液滴微流体测定法迅速获得普及，因为它们能够以高通量，高灵敏度，精确度和准确性分析生化反应，单个细胞或小细胞群。尽管如此，仍需要一种用户友好的低成本液滴分析技术。在本文中，我们通过开发两个液滴分析管道来满足这一需求免费的开源软件CellProfiler（CP）及其配套软件（CellProfiler Analyst，CPA）。为了说明该管线作为任何研究人员无需编程技能即可独立访问，可自由访问的液滴分析工具的能力，我们展示（i）使用单色图像对活荧光细菌进行液滴数字量化，以及（ii）分析具有包含不同化学成分的液滴的多色荧光图像。