A heterogeneous immunoassay is an efficient biomedical test. It aims to detect the presence of an analyte or to measure its concentration. It has many applications, such as manipulating particles and separating cancer cells from blood. The enhanced performance of immunosensors comes down to capturing more antigens with greater efficiency by antibodies in a short time. In this work, we report an efficient investigation of the effects of alternating current (AC) electrokinetic forces such as AC electroosmosis (ACEO), which arise when the fluid absorbs energy from an applied electric field, on the kinetics of the antigen-antibody binding in a flow system. The force can produce swirling structures in the fluid and, thus, improve the transport of the analyte toward the reaction surface of the immunosensor device. A numerical simulation is adequate for this purpose and may provide valuable information. The convection-diffusion phenomenon is coupled with the first-order Langmuir model. The governing equations are solved using the finite element method (FEM). The impact of AC electroosmosis on the binding reaction kinetics, the fluid flow stream modification, the analyte concentration diffusion, and the detection time of the biosensor under AC electroosmosis are analyzed.

中文翻译：

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交流电渗对微流体异质免疫测定效率的影响。

异质免疫测定是一种有效的生物医学测试。它旨在检测分析物的存在或测量其浓度。它具有许多应用程序，例如操纵粒子和从血液中分离癌细胞。免疫传感器性能的提高归结为可以在短时间内通过抗体以更高的效率捕获更多的抗原。在这项工作中，我们报告了交流电（AC）电动势，如交流电渗（ACEO）的有效研究，当流体从施加的电场吸收能量时，交流电电动势对抗原-抗体结合动力学的影响在流量系统中。该力可以在流体中产生涡旋结构，从而改善分析物向免疫传感器装置的反应表面的传输。数值模拟足以满足此目的，并且可以提供有价值的信息。对流扩散现象与一阶Langmuir模型耦合。控制方程使用有限元方法（FEM）求解。分析了交流电渗析对结合反应动力学，流体流变率，分析物浓度扩散以及生物传感器在交流电渗析下的检测时间的影响。