Coconut palm (Cocos nucifera L.) is rich in protein and consumed worldwide, coconut protein as a plant protein contains many nutrients and is beneficial for human health, but its allergic components also need to be considered. Therefore, further research on coconut protein is of great significance. In this study, nine proteins were identified through matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). Three proteins, namely, (1) 63 kDa globulin-like protein [Elaeis guineensis], (2) 63 kDa globulin-like protein [Phoenix dactylifera], and (3) glutelin type-A 3-like [E. guineensis] were initially found in coconut proteins. To further purify and identify the coconut protein, we used dialysate membrane and high-performance liquid chromatography-Ultraviole (HPLC-UV) to concentrate and separate the extracted protein. Two types of 63 kDa globulins were further purified and identified, namely, P. dactylifera and E. guineensis proteins with 564 and 398 amino acids, respectively, were identified. SWISS-MODEL was used to predict the tertiary structure of the 63 kDa globulins and showed that the structures of the coconut proteins were mostly similar to those of vicilin. This work provided a feasible and simple separation approach for the isolation and purification of coconut protein, and predicts the structure of newly identified protein.

可可椰子（Cocos nucifera L.）富含蛋白质，并且在世界范围内被消费。作为植物蛋白的椰子蛋白含有许多营养成分，对人体健康有益，但还需要考虑其过敏成分。因此，进一步研究椰子蛋白具有重要意义。在这项研究中，通过基质辅助激光解吸/电离串联飞行时间质谱（MALDI-TOF / TOF-MS）鉴定了9种蛋白质。三种蛋白质，分别是：（1）63 kDa的球蛋白样蛋白[ Elaeis guineensis ]，（2）63 kDa的球蛋白样蛋白[ Phoenix dactylifera ]和（3）谷蛋白A型3样[ E. guineensis最初发现于椰子蛋白中。为了进一步纯化和鉴定椰子蛋白，我们使用了透析液膜和高效液相色谱-乌特那韦（HPLC-UV）来浓缩和分离提取的蛋白。进一步纯化和鉴定了两种类型的63 kDa球蛋白，即分别鉴定了具有564和398个氨基酸的d。dactylifera和E. guineensis蛋白。SWISS-MODEL被用来预测63 kDa球蛋白的三级结构，并显示椰子蛋白的结构与vicilin的结构最相似。这项工作为椰子蛋白的分离和纯化提供了一种可行且简单的分离方法，并预测了新鉴定的蛋白的结构。