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Biomimetic human small muscular pulmonary arteries
Science Advances ( IF 13.6 ) Pub Date : 2020-03-25 , DOI: 10.1126/sciadv.aaz2598
Qianru Jin 1, 2 , Anil Bhatta 1, 2 , Jayson V. Pagaduan 1, 2 , Xing Chen 1, 2 , Hoku West-Foyle 3, 4 , Jiayu Liu 5 , Annie Hou 2, 6 , Dan Berkowitz 1, 6 , Scot C. Kuo 3, 4, 6 , Frederic B. Askin 7 , Thao D. Nguyen 5, 8 , David H. Gracias 2, 8 , Lewis H. Romer 1, 4, 6, 9, 10
Affiliation  

Changes in structure and function of small muscular arteries play a major role in the pathophysiology of pulmonary hypertension, a burgeoning public health challenge. Improved anatomically mimetic in vitro models of these microvessels are urgently needed because nonhuman vessels and previous models do not accurately recapitulate the microenvironment and architecture of the human microvascular wall. Here, we describe parallel biofabrication of photopatterned self-rolled biomimetic pulmonary arterial microvessels of tunable size and infrastructure. These microvessels feature anatomically accurate layering and patterning of aligned human smooth muscle cells, extracellular matrix, and endothelial cells and exhibit notable increases in endothelial longevity and nitric oxide production. Computational image processing yielded high-resolution 3D perspectives of cells and proteins. Our studies provide a new paradigm for engineering multicellular tissues with precise 3D spatial positioning of multiple constituents in planar moieties, providing a biomimetic platform for investigation of microvascular pathobiology in human disease.



中文翻译:

仿生人类小肌肉肺动脉

小肌肉动脉的结构和功能的变化在肺动脉高压的病理生理学中起着重要作用,而肺动脉高压是一个新兴的公共卫生挑战。由于非人类血管和先前的模型不能准确地概括人类微血管壁的微环境和结构,因此迫切需要这些微血管的改进的解剖学模拟体外模型。在这里,我们描述了可调大小和基础设施的光图案化自卷仿生肺动脉微血管的并行生物制造。这些微血管的特征是对齐的人类平滑肌细胞,细胞外基质和内皮细胞的解剖学精确分层和图案化,并显示出内皮寿命和一氧化氮生成的显着增加。计算图像处理产生了细胞和蛋白质的高分辨率3D透视图。我们的研究为工程化多细胞组织提供了一个新的范例,通过对平面部分中多个成分的精确3D空间定位,为研究人类疾病中微血管病理学提供了仿生平台。

更新日期:2020-03-26
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