Glyphosate is a widely used pesticide worldwide. The problem surrounding glyphosate is worth investigating, especially with its increased use, and an increasing number of studies have found that the toxic effect of glyphosate is objective. MiR-203 was seldom found in fish diseases or glyphosate researches. This article aims to explore the effect of miR-203 on carp lymphocytes during glyphosate exposure. Therefore, acridine orange/ethidium bromide (AO/EB) and flow cytometry were carried out to evaluate apoptosis, and we also detected CYPs (CYP1A1, CYP1B1, CYP1C), cytokine secretion (IL-1β, IL-8, IL-10, IFN-γ, TNF-α), inflammatory factors (NF-κB, cox-2), and the expression of miR-203 and the PI3K/AKT pathway by RT-PCR and Western blot analyses. Our results demonstrated that glyphosate exposure could induce lymphocyte apoptosis via regulation of miR-203 targeting of PI3K/AKT, which was accompanied by CYPs activation, abnormal cytokine expression and an inflammatory response. These results show that glyphosate is not nontoxic to fish and provide new insights for the usage of glyphosate as an herbicide in the future.

中文翻译：

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草甘膦通过调节鲤鱼（Cyprinus carpio L.）中PIK3R1的miR-203靶向作用诱导淋巴细胞功能障碍和凋亡。

草甘膦是全世界广泛使用的农药。草甘膦周围的问题值得研究，尤其是随着其使用量的增加，越来越多的研究发现草甘膦的毒性作用是客观的。在鱼类疾病或草甘膦研究中很少发现MiR-203。本文旨在探讨草甘膦暴露期间miR-203对鲤鱼淋巴细胞的影响。因此，进行了a啶橙/溴化乙锭（AO / EB）和流式细胞仪评估细胞凋亡，我们还检测了CYP（CYP1A1，CYP1B1，CYP1C），细胞因子分泌（IL-1β，IL-8，IL-10，通过RT-PCR和Western印迹分析确定IFN-γ，TNF-α，炎性因子（NF-κB，cox-2）以及miR-203和PI3K / AKT途径的表达。我们的结果表明，草甘膦暴露可通过调节miR-203靶向PI3K / AKT诱导淋巴细胞凋亡，并伴有CYPs激活，异常的细胞因子表达和炎症反应。这些结果表明草甘膦对鱼类不是无毒的，并为将来使用草甘膦作为除草剂提供了新的见解。