This work provided a comprehensive perspective to investigate the performance of NaHCO3-driving effect and mechanism including the antibiotic removal, degradation pathway and metabolites analysis, and the algal physiological response during the removal process. Cefuroxime sodium was selected as the target antibiotic. Our results showed that NaHCO3 did not facilitate self-decomposition of the target antibiotic, while drove the improvement on the removal capacity of every algal cell, which then attributed to the total removal efficiency. After 24 h, there was an improvement on the removal rate of the target antibiotic (from 10.21% to 92.89%) when NaHCO3 was added. The degradation pathway of the target antibiotic was confirmed by the formation of three main products (M1, M2 and M3), and the degradation process, that from M1 to M2 and M2 to M3, was accelerated by the existence of NaHCO3. Besides, a 4-stage model illustrated the relationship between NaHCO3 and antibiotic removal process. Moreover, algal culture that supplemented with NaHCO3 demonstrated a better growth capacity. A large increase in the content of chlorophyll a and a moderate increase in the activity of two carbon metabolic enzymes (RuBisCO and CA) might be viewed as a positive response of the algae during the NaHCO3-driving process.

中文翻译：

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NaHCO3去除藻类抗生素过程中依赖于过程的驱动模式的机理分析：效率，降解途径和代谢反应。

这项工作为研究NaHCO3-的驱动作用和机理（包括抗生素去除，降解途径和代谢物分析以及去除过程中的藻类生理反应）提供了全面的视角。选择头孢呋辛钠作为目标抗生素。我们的结果表明，NaHCO3不能促进目标抗生素的自分解，而推动了每个藻类细胞的去除能力的提高，这归因于总去除效率。24小时后，添加NaHCO3后目标抗生素的去除率有所提高（从10.21％增至92.89％）。通过三种主要产物（M1，M2和M3）的形成以及从M1到M2和M2到M3的降解过程，确定了目标抗生素的降解途径，NaHCO3的存在加速了这一过程。此外，一个4阶段模型说明了NaHCO3和抗生素去除过程之间的关系。此外，补充NaHCO3的藻类培养物显示出更好的生长能力。叶绿素a含量的大量增加和两种碳代谢酶（RuBisCO和CA）的活性的适度增加可能被视为NaHCO3驱动过程中藻类的阳性反应。