Supercritical anti-solvent precipitation (SAS) using carbon dioxide is a novel technique that can be used to produce powdered ingredients in small size particles, facilitating their incorporation into food matrices. In this work, the SAS precipitation of a licorice root ethanolic extract was studied. SAS assays were carried out at 15–20 MPa, 308.15 and 313.15 K, and two different concentrations (9.6 and 14.2 mg/ml) of the ethanolic licorice extract. In the range of conditions investigated, SAS pressure and temperature did not affect significantly the precipitation yield, but phytochemicals recovery was higher with the lower licorice extract concentration. Moreover, the fractionation of licorice bioactives (liquiritin, liquiritigenin, isoliquiritigenin, glabridin and glycyrrhizic acid) was assessed, together with the content of total phenolic compounds and antioxidant activity of the powders and oleoresin by-products obtained. In this respect, precipitates and oleoresins presented significant differences in the concentration of some licorice bioactives, and higher antioxidant activity was observed in precipitates. Additionally, significant effect of pressure, temperature and licorice extract concentration on the morphology and particle size of precipitates was observed, recovering smaller and more regular particles at 15–20 MPa, 313.15 K and 9.6 mg/ml licorice extract concentration, attaining satisfactory yield and antioxidant activity.

使用二氧化碳的超临界抗溶剂沉淀（SAS）是一种新技术，可用于生产小颗粒的粉状成分，从而有助于将其掺入食品基质中。在这项工作中，研究了甘草根乙醇提取物的SAS沉淀。SAS分析在15–20 MPa，308.15和313.15 K以及两种不同浓度（9.6和14.2 mg / ml）的甘草乙醇提取物中进行。在所研究的条件范围内，SAS压力和温度不会显着影响沉淀产量，但甘草提取物浓度较低时，植物化学物质的回收率较高。此外，还评估了甘草生物活性成分（liquiritin，liquiritigenin，isoliquiritigenin，glabridin和甘草次酸）的分离度，以及总酚类化合物的含量以及粉末和油性树脂副产物的抗氧化活性。在这方面，沉淀物和油树脂在某些甘草生物活性物质的浓度上存在显着差异，并且在沉淀物中观察到更高的抗氧化活性。此外，观察到压力，温度和甘草提取物浓度对沉淀物的形态和粒径有显着影响，在15–20 MPa，313.15 K和9.6 mg / ml甘草提取物浓度下回收更小且更规则的颗粒，获得令人满意的收率和抗氧化活性。在沉淀物中观察到更高的抗氧化活性。此外，观察到压力，温度和甘草提取物浓度对沉淀物的形态和粒径有显着影响，在15–20 MPa，313.15 K和9.6 mg / ml甘草提取物浓度下回收更小，更规则的颗粒，获得令人满意的收率和抗氧化活性。在沉淀物中观察到更高的抗氧化活性。此外，观察到压力，温度和甘草提取物浓度对沉淀物的形态和粒径有显着影响，在15–20 MPa，313.15 K和9.6 mg / ml甘草提取物浓度下回收更小，更规则的颗粒，获得令人满意的收率和抗氧化活性。