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Profiling of pentacyclic triterpenes and polyphenols by LC-MS in Arbequina and Empeltre table olives
LWT - Food Science and Technology ( IF 6 ) Pub Date : 2020-03-25 , DOI: 10.1016/j.lwt.2020.109310
Rocío Moreno-González , M. Emília Juan , Joana M. Planas

Pentacyclic triterpenes (PT) and polyphenols from two varieties of table olives, Arbequina and Empeltre, were determined using a unique extraction from the same olive samples prior to their analysis by LC-APCI-MS and LC-ESI-MS/MS, respectively. Validation provided linear correlation and high sensitivity, as well as adequate precision and accuracy (RSD < 15.0%). The analysis of these table olives allowed the identification and quantification of 3 PT and 16 polyphenols. The most abundant were PT, with a total content of 3.28 g/kg in Arbequina and 2.77 g/kg in Empeltre, ahead of 1.04 g/kg and 0.83 g/kg of polyphenols, respectively. The main PT was maslinic acid, with 2.51 g/kg in Arbequina and 1.86 g/kg in Empeltre, followed by oleanolic acid and finally, erythrodiol. The main polyphenol was hydroxytyrosol, with 0.51 g/kg in Arbequina and 0.39 g/kg in Empeltre, succeeded by luteolin and verbascoside. Bioactive compounds were found in concentrations high enough to exert beneficial effects on health, thus suggesting that a regular consumption of table olives, in the context of a healthy lifestyle, could contribute to the prevention of chronic diseases.



中文翻译:

通过LC-MS在Arbequina和Empeltre食用橄榄中分析五环三萜和多酚

分别使用LC-APCI-MS和LC-ESI-MS / MS对同一橄榄样品进行了独特的提取,确定了来自两种食用橄榄Arbequina和Empeltre的五环三萜(PT)和多酚。验证提供了线性相关性和高灵敏度,以及足够的精度和准确性(RSD <15.0%)。通过对这些食用橄榄的分析,可以鉴定和定量3 PT和16种多酚。PT含量最高,在Arbequina和Empeltre中的总含量分别为3.28 g / kg和2.77 g / kg,分别领先于多酚的1.04 g / kg和0.83 g / kg。主要的PT是山梨酸,在Arbequina中为2.51 g / kg,在Empeltre中为1.86 g / kg,其次是齐墩果酸,最后是赤藓糖醇。主要的多酚是羟基酪醇,在Arbequina中为0.51 g / kg,0。在Empeltre中为39 g / kg,随后是木犀草素和马齿bas甙。发现生物活性化合物的浓度足以对健康产生有益影响,因此表明,在健康的生活方式中经常食用食用橄榄可有助于预防慢性疾病。

更新日期:2020-03-26
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