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Hyperthermia affects collagen fiber architecture and induces apoptosis in pancreatic and fibroblast tumor hetero-spheroids in vitro.
Nanomedicine: Nanotechnology, Biology and Medicine ( IF 5.4 ) Pub Date : 2020-03-25 , DOI: 10.1016/j.nano.2020.102183
Susann Piehler 1 , Lena Wucherpfennig 1 , Felista L Tansi 1 , Alexander Berndt 2 , Rainer Quaas 3 , Ulf Teichgraeber 1 , Ingrid Hilger 1
Affiliation  

Desmoplasia, an aberrant production of extracellular matrix (ECM), is considered as one predictive marker of malignancy of pancreatic cancer. In this paper, we study the effect of mild hyperthermia on fibrillary collagen architecture in murine Achilles tendons and in a pancreatic cancer model, in vitro, i.e. 3D hetero-type tumor spheroids, consisting of pancreatic cancer (Panc-1) cells and fibroblasts (WI-38), producing collagen fibers. We clearly demonstrate that i) mild hyperthermia (40 °C, 42 °C) damages the collagen architecture in murine Achilles tendons. ii) Mild extrinsic (hot air) and iron oxide nanoparticle based magnetic hyperthermia reduce the level of collagen fiber architecture in the generated hetero-type tumor spheroids. iii) Mild magnetic hyperthermia reduces cell vitality mainly through apoptotic and necrotic processes in the generated tumor spheroids. In conclusion, hetero-type 3D tumor spheroids are suitable for studying the effect of hyperthermia on collagen fibers, in vitro.



中文翻译:

体温过高会影响胶原纤维的结构,并在体外诱导胰腺和成纤维细胞肿瘤异球体的凋亡。

异形增生是细胞外基质(ECM)的异常产生,被认为是胰腺癌恶性程度的一种预测指标。在本文中,我们研究了轻度高温对鼠跟腱和胰腺癌模型中纤维状胶原结构的影响,即体外模型,即由胰腺癌(Panc-1)细胞和成纤维细胞组成的3D异型肿瘤球体( WI-38),生产胶原纤维。我们清楚地证明,i)温和的高温(40°C,42°C)会破坏小鼠跟腱的胶原结构。ii)温和的外在(热空气)和基于氧化铁纳米粒子的磁热疗会降低所产生的异型肿瘤球体中胶原纤维结构的水平。iii)轻度的磁热疗主要通过所产生的肿瘤球体的凋亡和坏死过程降低细胞活力。总之,异型3D肿瘤球体适合于体外研究热疗对胶原纤维的影响。

更新日期:2020-03-25
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