Considering the nutritional benefits of fruits on human health, this study aimed to screen 11 fruits found in the Brazilian Savanna for glycoside hydrolases inhibitors with antioxidant and anti-glycation properties. Aqueous and ethanol extracts of pulps were used in enzymatic assays to evaluate the inhibitory potential against α-amylase and α-glucosidase, as well as antioxidant (2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays) and anti-glycation (BSA-fructose model) capacities. Ethanol extracts of Campomanesia xanthocarpa, Byrsonima verbascifolia and Eugenia dysenterica had the lowest DPPH IC₅₀ values (<0.25 mg/ml), while ethanol extracts of Genipa americana and B. verbascifolia had the highest ORAC values (>0.60 mmol trolox equivalents/g). The extracts of E. dysenterica showed potential to inhibit glycation (IC₅₀ < 0.40 mg/ml) and α-glucosidase (IC₅₀ < 20 μg/ml). Liquid-liquid fractionation of the extracts of E. dysenterica fruit pulp resulted in the ethyl acetate fraction showing higher antioxidant (DPPH IC₅₀ 10 μg/ml and ORAC 1.3 mmol trolox equivalents/g) and anti-glycation (IC₅₀ 0.10 mg/ml) capacities, and α-glucosidase inhibition activity (IC₅₀ 11 μg/ml). HPLC-ESI-MS/MS analysis suggested the presence of antioxidant molecules in E. dysenterica fruit pulp, such as ferulic and gallic acids, myricetin, quercetin and kaempferol-pentosides. Non-conventional fruits found in the Brazilian Savanna, a global biodiversity hotspot, with antioxidant and anti-glycation properties, could be potential sources of nutraceuticals to combat oxidative stress, which is present in many pathologies. In addition, the capacity of E. dysenterica to inhibit α-glucosidase and non-enzymatic glycation makes it especially useful for in vivo studies related to diabetes mellitus.

考虑到水果对人体健康的营养益处，本研究旨在筛选巴西大草原中的11种水果中具有抗氧化和抗糖基化特性的糖苷水解酶抑制剂。纸浆的水提取物和乙醇提取物用于酶法分析，以评估其对α-淀粉酶和α-葡萄糖苷酶以及抗氧化剂（2,2-二苯基-1-picylhydrazyl（DPPH））和氧自由基吸收能力（ORAC）的抑制潜力分析方法）和抗糖化功能（BSA-果糖模型）。的乙醇提取物Campomanesia xanthocarpa，Byrsonima verbascifolia和尤金尼亚dysenterica具有最低的DPPH IC _50个值（<0.25毫克/毫升），而乙醇提取物美洲格尼帕树和桔梗芽孢杆菌的ORAC值最高（> 0.60 mmol trolox当量/ g）。痢疾肠杆菌的提取物显示出抑制糖基化（IC ₅₀  <0.40 mg / ml）和α-葡萄糖苷酶（IC ₅₀  <20μg/ ml）的潜力。痢疾肠杆菌果肉提取物的液-液分馏导致乙酸乙酯级分显示出更高的抗氧化剂（DPPH IC ₅₀ 10μg/ ml和ORAC 1.3 mmol trolox当量/ g）和抗糖化作用（IC ₅₀ 0.10 mg / ml）容量和α-葡萄糖苷酶抑制活性（IC ₅₀ 11μg/ ml）。HPLC-ESI-MS / MS分析表明痢疾肠杆菌中存在抗氧化剂分子水果果肉，例如阿魏酸和没食子酸，杨梅素，槲皮素和山奈酚-戊糖苷。在全球广泛的生物多样性热点巴西热带稀树草原发现的非常规水果，具有抗氧化和抗糖基化特性，可能是多种抗氧化应激的营养保健品的潜在来源，而这种营养品在许多病理中都存在。此外，容量E. dysenterica抑制α葡糖苷酶和非酶糖基化使得它是特别有用的体内与糖尿病相关的研究，糖尿病。