A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.,Molecular and Cellular Probes

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A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.
Molecular and Cellular Probes ( IF 3.3 ) Pub Date : 2020-03-25 , DOI: 10.1016/j.mcp.2020.101564
Kan-Kan Yang ₁ , Dong-Dong Yin ₁ , Liang Xu ₁ , Yue-Qiao Liang ₁ , Jian Tu ₁ , Xiang-Jun Song ₁ , Ying Shao ₁ , Hong-Mei Liu ₁ , Ke-Zong Qi ₁

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Goose circovirus (GoCV) is a potential immunosuppressive virus that poses a great hazard to the goose industry and has been shown to be widely distributed throughout China. We have established a fast, sensitive and highly specific TaqMan real-time quantitative PCR detection method for this virus. Specific primers and probes were designed against the conserved regions of the genomic GoCV Rep gene. The results showed that the assay was highly specific and sensitive for GoCV and did not cross-react with other non-targeted waterfowl viruses. The established method will be helpful for epidemiological detection and may be effective in the prevention and control of the disease.

更新日期：2020-03-25

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