Plant glycosides are of great interest for industries. Glycosylation of plant secondary metabolites can greatly improve their solubility, biological activity, or stability. This allows some plant glycosides to be used as food additives, cosmetic products, health products, antisepsis and anti-cancer drugs. With the continuous expansion of market demand, a variety of biological fermentation technologies has emerged. This review focuses on recombinant microbial biosynthesis of plant glycosides, which uses UDP-sugars as precursors, and summarizes various strategies to increase the yield of glycosides with a key concentration on UDP-sugar supply based on four aspects, i.e., gene overexpression, UDP-sugar recycling, mixed fermentation, and carbon co-utilization. Meanwhile, the application potential and advantages of various techniques are introduced, which provide guidance to the development of high-yield strains for recombinant microbial production of plant glycosides. Finally, the technical challenges of glycoside biosynthesis are pointed out with discussions on future directions of improving the yield of recombinantly synthesized glycosides.

植物糖苷对工业非常感兴趣。植物次生代谢产物的糖基化可以大大提高其溶解度，生物活性或稳定性。这使得某些植物糖苷可用作食品添加剂，化妆品，保健品，防腐剂和抗癌药。随着市场需求的不断扩大，出现了多种生物发酵技术。这篇综述集中于使用UDP糖作为前体的植物糖苷的重组微生物生物合成，并基于四个方面，即基因过表达，UDP-糖回收，混合发酵和碳联合利用。同时，介绍了各种技术的应用潜力和优势，它们为开发用于植物糖苷的重组微生物生产的高产菌株提供了指导。最后，通过对提高重组合成糖苷产量的未来方向的讨论，指出了糖苷生物合成的技术挑战。