BACKGROUND To explore the mechanism of action of Tripterygium wilfordii Hook (TWH) in the treatment of Crohn's disease (CD) by network pharmacology. METHODS Traditional Chinese Medicine Systems Pharmacology database and analysis platform (TCMSP) was used to obtain the active constituents and targets of TWH. "Crohn's disease" was used as a search term to search for related targets of CD from GeneCards database and OMIM database, thereby obtaining the targets of TWH against CD. The Cytoscape 3.7.1 software was used to construct a Chinese medicine compound-target network and STRING database to construct a protein-protein interaction network (PPI). The DAVID 6.8 online tool was used to perform gene ontology (GO) and kyoto encyclopedia of genes and genome (KEGG) pathway enrichment analysis of overlapping targets. RESULTS The database results showed that there were 30 active ingredients (14 key active ingredients) in TWH and 36 targets were screened out for CD treatment. Network analysis indicated that main targets of main active components of TWH were target genes such as VEGFA, MAPK8 and CASP3, which are involved in the regulation of cancer pathway, TNF signal pathway, hepatitis B pathway, apoptosis pathway, NF-kappa B signal pathway and so forth. CONCLUSIONS TWH can play a multi-target and multi-channel synergistic treatment of CD by anti-angiogenesis, anti-apoptosis, anti-inflammation and immune regulation.

中文翻译：

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雷公藤多形钩治疗克罗恩病的网络药理研究。

背景技术通过网络药理学探讨雷公藤（TWH）在克罗恩病（CD）治疗中的作用机制。方法采用中药系统药理数据库和分析平台（TCMSP）获得TWH的活性成分和靶标。使用“克罗恩氏病”作为搜索词，从GeneCards数据库和OMIM数据库中搜索CD的相关靶标，从而获得针对CD的TWH靶标。使用Cytoscape 3.7.1软件构建中药化合物-靶标网络，并使用STRING数据库构建蛋白质-蛋白质相互作用网络（PPI）。DAVID 6.8在线工具用于执行基因本体论（GO）和基因京都百科全书以及重叠靶标的基因组（KEGG）途径富集分析。结果数据库结果表明，TWH中有30种有效成分（14种关键活性成分），并筛选出36种靶标用于CD治疗。网络分析表明，TWH主要活性成分的主要靶标是靶基因，如VEGFA，MAPK8和CASP3，它们参与了癌症通路，TNF信号通路，乙型肝炎通路，细胞凋亡通路，NF-κB信号通路的调控。等等。结论TWH可通过抗血管生成，抗凋亡，抗炎和免疫调节作用来对CD进行多靶点和多通道协同治疗。网络分析表明，TWH主要活性成分的主要靶标是靶基因，如VEGFA，MAPK8和CASP3，它们参与了癌症通路，TNF信号通路，乙型肝炎通路，细胞凋亡通路，NF-κB信号通路的调控。等等。结论TWH可通过抗血管生成，抗凋亡，抗炎和免疫调节作用来对CD进行多靶点和多通道协同治疗。网络分析表明，TWH主要活性成分的主要靶标是靶基因，如VEGFA，MAPK8和CASP3，它们参与了癌症通路，TNF信号通路，乙型肝炎通路，细胞凋亡通路，NF-κB信号通路的调控。等等。结论TWH可通过抗血管生成，抗凋亡，抗炎和免疫调节作用来对CD进行多靶点和多通道协同治疗。