The Rumex acetosa has been used in medicinal treatment, food technology and phytotherapeutics in Eastern Asia and many other countries. However, its effect on cardiovascular system and antiplatelet activity remained to be known. In this study, we examined the antiplatelet activity of R. acetosa in detailed manner to understand underlying mechanism. To study this, whole blood was obtained from male Sprague Dawley (SD) rats and aggregation of washed platelets measured using light transmission aggregometry. Intracellular calcium ion concentration ([Ca2+]i) was measured using Fura-2/AM while ATP release evaluated by luminometer. Activation of integrin αIIbβ3 analyzed by flow cytometry and clot retraction. Furthermore, we studied the signaling pathways mediated by R. acetosa extract by western blot analysis. R. acetosa extract markedly inhibited collagen-induced platelet aggregation and ATP release in a dose-dependent manner. It also suppressed [Ca2+]i mobilization, integrin αIIbβ3 activation and clot retraction. The extract significantly attenuated phosphorylation of the MAPK pathway (i.e., ERK1/2, JNK), MKK4, PI3K/Akt, and Src family kinase. Taken together, this data suggests that R. acetosa extract exhibits anti-platelet activity via modulating MAPK, PI3K/Akt pathways, and integrin αIIbβ3-mediated inside-out and outside-in signaling, and it may protect against the development of platelet-related cardiovascular diseases.

中文翻译：

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鼠尾草Rumex acetosa调节大鼠血小板功能并抑制血栓形成。

Rumex acetosa已在东亚和许多其他国家用于药物治疗，食品技术和植物治疗学。但是，其对心血管系统和抗血小板活性的影响尚不清楚。在这项研究中，我们以详细的方式检查了乙酰丙酮杆菌的抗血小板活性，以了解其潜在机制。为了研究这一点，从雄性Sprague Dawley（SD）大鼠获得全血，并使用光透射聚集法测量洗过的血小板的聚集。使用Fura-2 / AM测量细胞内钙离子浓度（[Ca 2+] i），同时通过发光计评估ATP释放。通过流式细胞仪和血块收回分析整联蛋白αIIbβ3的激活。此外，我们通过蛋白质印迹分析研究了乙酰丙酮酸提取物介导的信号通路。R. 乙酰丙酮提取物以剂量依赖性方式显着抑制胶原诱导的血小板聚集和ATP释放。它还抑制了[Ca2 +] i的动员，整联蛋白αIIbβ3的激活和血凝块的回缩。提取物显着减弱了MAPK途径（即ERK1 / 2，JNK），MKK4，PI3K / Akt和Src家族激酶的磷酸化。综上所述，该数据表明醋母提取物通过调节MAPK，PI3K / Akt途径和整联蛋白αIIbβ3介导的由内而外由内而外的信号传导表现出抗血小板活性，并且可以防止血小板相关性的发展。心血管疾病。提取物显着减弱了MAPK途径（即ERK1 / 2，JNK），MKK4，PI3K / Akt和Src家族激酶的磷酸化。综上所述，该数据表明醋母提取物通过调节MAPK，PI3K / Akt途径和整联蛋白αIIbβ3介导的由内而外由内而外的信号传导表现出抗血小板活性，并且可以防止血小板相关性的发展。心血管疾病。提取物显着减弱了MAPK途径（即ERK1 / 2，JNK），MKK4，PI3K / Akt和Src家族激酶的磷酸化。综上所述，该数据表明醋母提取物通过调节MAPK，PI3K / Akt途径和整联蛋白αIIbβ3介导的由内而外由内而外的信号传导表现出抗血小板活性，并且可以防止血小板相关性的发展。心血管疾病。