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Enhancing Enzyme-Mediated Hydrolysis of Mechanical Pulps by Deacetylation and Delignification
ACS Sustainable Chemistry & Engineering ( IF 8.4 ) Pub Date : 2020-03-24 , DOI: 10.1021/acssuschemeng.9b07226
Jie Wu 1 , Richard P. Chandra 1 , Kwang Ho Kim 2 , Chang Soo Kim 2 , Yunqiao Pu 3 , Arthur J. Ragauskas 3, 4 , Jack Nicholas Saddler 1
Affiliation  

Alkaline induced deacetylation of the hemicellulose combined with subsequent mechanical refining enhanced the enzyme-mediated hydrolysis of pretreated corn stover. The addition of either NaOH (80 °C) or mild KOH (25 °C) to corn stover prior to mechanical refining led to greater than 80% deacetylation with the NaOH treatment also solubilizing low-molecular-weight lignin that was enriched in β-O-4 linkages with more than 25% and 13% of the total and surface lignin removed, respectively. The influence of deacetylation and delignification were further enhanced when NaOH was supplemented with 3% Na2SO3, resulting in 100% deacetylation, 34% delignification, and a >20% increase in the hydrolysis yield of the substrate xylan. A milder KOH treatment resulted in the retention of more than 95% of the lignin within the cellulose rich, water-insoluble fraction with no apparent change in the surface lignin. However, both methods resulted in enhanced xylan hydrolysis when treated with xylanases, suggesting that deacetylation had enhanced accessibility to the xylan present in the pretreated of corn stover. It was apparent that cellulose accessibility was also enhanced by partial delignification, as NaOH treatment resulted in a 65% and 43% increase in the Water Retention Value and Directed Orange dye adsorption, respectively.

中文翻译:

通过脱乙酰化和木质素化增强酶介导的机械纸浆水解

碱性诱导的半纤维素脱乙酰基结合随后的机械精制增强了酶促预处理的玉米秸秆的水解。在机械精炼之前向玉米秸秆中添加NaOH(80°C)或温和的KOH(25°C)会导致NaOH处理的脱乙酰化率超过80%,这也可以溶解富含β-淀粉的低分子量木质素O-4键合分别去除了总木质素和表面木质素的25%和13%以上。NaOH加入3%Na 2 SO 3时,脱乙酰化和去木质素的影响进一步增强。,导致100%的脱乙酰基作用,34%的脱木素作用以及底物木聚糖的水解产率提高> 20%。较温和的KOH处理导致富纤维素的水不溶级分中保留了95%以上的木质素,而表面木质素没有明显变化。然而,当用木聚糖酶处理时,两种方法均导致木聚糖水解增强,表明脱乙酰基具有增强的对玉米秸秆预处理中存在的木聚糖的可及性。显然,通过部分去木质素作用还提高了纤维素的可及性,因为NaOH处理分别使保水值和定向橙染料的吸附分别增加了65%和43%。
更新日期:2020-04-23
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