当前位置: X-MOL 学术ChemBioChem › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The Influence of Competing tRNA Abundance on Translation: Quantifying the Efficiency of Sense Codon Reassignment at Rarely Used Codons.
ChemBioChem ( IF 3.2 ) Pub Date : 2020-03-23 , DOI: 10.1002/cbic.202000052
David G Schwark 1 , Margaret A Schmitt 1 , Wil Biddle 1 , John D Fisk 1
Affiliation  

Making sense: We quantitatively evaluate the effect of the abundance of competing endogenous tRNAs on the ability of orthogonal tRNA variants to reassign E. coli sense codons. For rarely used codons, a strong correlation exists between tRNA abundance and reassignment efficiency. Our measurements suggest that the Ile AUA codon is a particularly good target for genetic code expansion.
image


中文翻译:

竞争性tRNA丰度对翻译的影响:量化稀有密码子上有义密码子重新分配的效率。

有意义:我们定量评估竞争性内源性tRNA的丰富度对正交tRNA变体重新分配大肠杆菌有义密码子的能力的影响。对于很少使用的密码子,tRNA丰度与重新分配效率之间存在很强的相关性。我们的测量结果表明,Ile AUA密码子是遗传密码扩展的一个特别好的目标。
图片
更新日期:2020-03-23
down
wechat
bug