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Evaluation of the effects of different intensities of forced running wheel exercise on oxidative stress biomarkers in muscle, liver and serum of untrained rats.
Lab Animal ( IF 6.9 ) Pub Date : 2020-03-23 , DOI: 10.1038/s41684-020-0503-7
Seyed Reza Mousavi 1 , Mahvash Jafari 2 , Sajjad Rezaei 3 , Hamid Agha-Alinejad 3 , Vahid Sobhani 2
Affiliation  

Exercise induces different effects on antioxidant status depending on its intensity. The forced running wheel (FRW) model maintains a constant intensity and volume during exercise. The aim of the present study was to investigate the effects of FRW exercise at different running speeds on several serum biochemical parameters of liver and muscle functions and on oxidative stress biomarkers in skeletal muscle, liver and serum in the rat. Thirty-six male Wistar rats were randomly divided into six groups. Five groups participated in constant power tests at intensities of 10, 13, 14.5, 16, and 17.5 m/min, and a non-exercise group was chosen as the control. Serum, muscle and liver tissues were collected after the tests and analyzed. At speeds >16 m/min, exercise on an FRW significantly increased several serum biochemical parameters, malondialdehyde level and superoxide dismutase activity in all tissues of exercise rats compared with control rats; FRW exercise also increased catalase activity in the liver and glutathione S-transferase activity in muscle, whereas it decreased glutathione level in all tissues and catalase activity in muscle and serum. These data suggest that FRW exercise in rats activates an adaptation of the antioxidant system response in skeletal muscle at speeds <16 m/min, whereas it induces oxidative stress at higher speeds in muscle, liver and serum. In addition, we observed a correlation between the systematic and local oxidative stress status in rats after exercise on FRW.



中文翻译:

评价不同强度的轮转运动对未训练大鼠肌肉,肝脏和血清中氧化应激生物标志物的影响。

锻炼会根据其强度而对抗氧化剂状态产生不同的影响。强制行走轮(FRW)模型在运动过程中保持恒定的强度和音量。本研究的目的是研究FRW运动在不同的速度下对大鼠肝脏和肌肉功能的几个血清生化参数以及对大鼠骨骼肌,肝脏和血清中氧化应激生物标志物的影响。将三十六只雄性Wistar大鼠随机分为六组。五个组以10、13、14.5、16和17.5 m / min的强度参加恒定功率测试,并选择一个非运动组作为对照组。测试后收集血清,肌肉和肝组织并进行分析。在> 16 m / min的速度下,进行FRW锻炼会显着增加一些血清生化参数,与对照组相比,运动大鼠所有组织的丙二醛水平和超氧化物歧化酶活性;FRW运动还可以增加肝脏和谷胱甘肽中的过氧化氢酶活性肌肉中的S-转移酶活性,但会降低所有组织中的谷胱甘肽水平以及肌肉和血清中的过氧化氢酶活性。这些数据表明,在大鼠中进行FRW运动时,速度<16 m / min时,骨骼肌中抗氧化系统反应的适应性得到了激活,而在肌肉,肝脏和血清中,它以较高的速度诱导了氧化应激。此外,我们观察到FRW运动后大鼠的系统性氧化应激和局部氧化应激状态之间存在相关性。

更新日期:2020-04-24
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