The treatment of landfill leachate could be challenging for the biological wastewater treatment systems due to its high toxicity and the presence of poorly biodegradable contaminants. In this study, the bioaugmentation technology was successfully applied in sequencing batch reactors (SBRs) fed with the phenolic landfill leachate by inoculation of the activated sludge (AS) with two phenol-degrading Pseudomonas putida OR45a and Pseudomonas putida KB3 strains. According to the results, the SBRs bioaugmented with Pseudomonas strains withstood the increasing concentrations of the leachate. This resulted in the higher removal efficiency of the chemical oxygen demand (COD) of 79–86%, ammonia nitrogen of 87–88% and phenolic compounds of 85–96% as compared to 45%, 64%, and 50% for the noninoculated SBR. Simultaneously, the bioaugmentation of the AS allowed to maintain the high enzymatic activity of dehydrogenases, nonspecific esterases, and catalase in this ecosystem, which contributed to the higher functional capacity of indigenous microorganisms than in the noninoculated AS. Herein, the stress level experienced by the microorganisms in the SBRs fed with the leachate computed based on the cellular ATP measurements showed that the abundance of exogenous Pseudomonas strains in the bioreactors contributed to the reduction in effluent toxicity, which was reflected by a decrease in the stress biomass index to 32–45% as compared to the nonbioaugmented AS (76%).

中文翻译：

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用酚类垃圾渗滤液补料的序批式反应器中恶臭假单胞菌 OR45a 和恶臭假单胞菌 KB3 的生物强化潜力分析

由于垃圾渗滤液的高毒性和难生物降解污染物的存在，垃圾渗滤液的处理对生物废水处理系统来说可能具有挑战性。在这项研究中，生物强化技术成功地应用于以酚类垃圾渗滤液为原料的序批式反应器（SBR），通过在活性污泥（AS）中接种两种降解苯酚的恶臭假单胞菌 OR45a 和恶臭假单胞菌 KB3 菌株。根据结果​​，用假单胞菌菌株进行生物强化的 SBR 能够承受不断增加的渗滤液浓度。这导致化学需氧量 (COD) 的去除效率更高，为 79-86%，氨氮为 87-88%，酚类化合物为 85-96%，相比之下，45%、64% 和 50%未接种的 SBR。同时地，AS 的生物强化允许在该生态系统中保持脱氢酶、非特异性酯酶和过氧化氢酶的高酶活性，这有助于本地微生物比未接种的 AS 具有更高的功能能力。在此，基于细胞 ATP 测量计算的渗滤液喂养的 SBR 中微生物所经历的压力水平表明，生物反应器中外源性假单胞菌菌株的丰度有助于降低流出物毒性，这反映在与非生物增强 AS (76%) 相比，应激生物量指数提高到 32-45%。这有助于本地微生物比未接种的 AS 具有更高的功能能力。在此，基于细胞 ATP 测量计算的渗滤液喂养的 SBR 中微生物所经历的压力水平表明，生物反应器中外源性假单胞菌菌株的丰度有助于降低流出物毒性，这反映在与非生物增强 AS (76%) 相比，应激生物量指数提高到 32-45%。这有助于本地微生物比未接种的 AS 具有更高的功能能力。在此，基于细胞 ATP 测量计算的渗滤液喂养的 SBR 中微生物所经历的压力水平表明，生物反应器中外源性假单胞菌菌株的丰度有助于降低流出物毒性，这反映在与非生物增强 AS (76%) 相比，应激生物量指数提高到 32-45%。