Liquid marble (LM), a non-stick drop coated with micro- or nano-scale particles, has great potential in a wide range of applications. LMs have an advantageous feature in which gas or vapor can freely transport through their particle shell; therefore, it makes them an ideal candidate to be utilized as microbioreactor containing aerobic microorganisms. In this study, safer and more biocompatible LMs were successfully prepared using a food-grade calcium stearate microparticle as a stabilizer. As the volume of core liquid increased, the height of LM increased and reached a constant value, as a similar trend has been reported in conventional LMs. The drying rate curve of the LMs confirmed that the LMs have a similar pattern with the drying of typical wet powders. The drying rate depended on the salt species in the core solution and the environmental humidity. For instance, in the case of MgCl₂, by changing humidity from 40 to 80% RH, the lifetime of LMs (time in which the LM dried completely) was increased to about 900 min. This is nearly three times longer than those have no salt and at 40% RH. Model aerobic bacteria Bacillus subtilis has actively proliferated inside the LM during 24-h incubation. Comparing with the test tube cultivations under O₂-rich stationary or O₂ rich–shaken conditions, the cultivation in the LM system showed a higher proliferation than the test tube systems. As a conclusion, we demonstrated that the calcium stearate LM system would be an ideal candidate for safer and easily available microbioreactor containing aerobic bacteria.

液态大理石（LM）是一种不粘液滴，涂有微米级或纳米级的颗粒，在广泛的应用领域中具有巨大的潜力。LM具有一个有利的特征，即气体或蒸气可以自由地通过其粒子壳传输；因此，它使其成为含有需氧微生物的微生物反应器的理想候选者。在这项研究中，使用食品级硬脂酸钙微粒作为稳定剂成功地制备了更安全，更具生物相容性的LM。随着核心液体体积的增加，LM的高度增加并达到一个恒定值，因为传统LM中已经报道了类似的趋势。LM的干燥速率曲线证实，LM具有与典型的湿粉的干燥相似的模式。干燥速率取决于芯溶液中的盐种类和环境湿度。例如，对于MgCl_{如图2所示}，通过将湿度从40％RH改变为80％，LM的寿命（LM完全干燥的时间）增加到约900分钟。这是在40％相对湿度下几乎不含盐的三倍。需氧菌模型枯草芽孢杆菌在24小时的孵育过程中已在LM内部积极增殖。与在富含O _2的固定或充满O ₂的摇动条件下的试管培养相比，LM系统中的培养显示出比试管系统更高的增殖。结论是，我们证明了硬脂酸钙LM系统将是更安全，更容易获得的含有好氧细菌的微生物反应器的理想选择。