Abstract

Objectives

To investigate the viscosity values of mixtures of hyaluronic acids with different molecular weights and the effects of these mixtures on the enzymatic activities of lysozyme and peroxidase.

Materials and methods

Mixtures of high molecular weight (1 or 2 MDa) and low molecular weight (10 or 100 kDa) hyaluronic acids at different concentrations were used for viscosity measurements. Hyaluronic acid mixtures showing viscosity values similar to those of human whole saliva were used for enzyme experiments in solution and on hydroxyapatite surface. Hen egg-white lysozyme, bovine lactoperoxidase, and human whole saliva were used as enzyme sources. Lysozyme activity was measured by hydrolysis of fluorescein-labeled Micrococcus lysodeikticus. Peroxidase activity was measured by oxidation of fluorogenic 2′,7′-dichlorofluorescein to fluorescing 2′,7′-dichlorofluorescein.

Results

The mixtures of 1 MDa (0.5 mg/mL) or 2 MDa (0.2 mg/mL) hyaluronic acid with 10 kDa (2.0 mg/mL) or 100 kDa (0.1 mg/mL) hyaluronic acid had viscosity values similar to those of human whole saliva at shear rates, reflecting normal oral functions. Compared with single molecular weight hyaluronic acids, these mixtures showed viscosity values more similar to those of human whole saliva. The mixtures inhibited lysozyme and peroxidase activities on the hydroxyapatite surfaces; however, the degree of inhibition did not differ from that of hyaluronic acid of 1 or 2 MDa only.

Conclusions

Compared with single molecular weight hyaluronic acids, hyaluronic acid mixtures showed viscosity values more similar to those of human whole saliva, without additional inhibitory effects on lysozyme and peroxidase activities.

Clinical relevance

Hyaluronic acid mixtures offer distinct advantages for the development of saliva substitutes.

中文翻译：

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不同分子量透明质酸混合物的粘度及其对溶菌酶和过氧化物酶活性的影响

摘要

目标

研究具有不同分子量的透明质酸混合物的粘度值，以及这些混合物对溶菌酶和过氧化物酶的酶活性的影响。

材料和方法

使用不同浓度的高分子量（1或2 MDa）和低分子量（10或100 kDa）透明质酸的混合物进行粘度测量。在溶液中和羟基磷灰石表面上使用的透明质酸混合物的粘度值与人类整个唾液的粘度值相似，用于酶实验。鸡卵清溶菌酶，牛乳过氧化物酶和人全唾液用作酶源。溶菌酶活性通过荧光素标记的溶菌微球菌的水解来测量。通过将荧光的2'，7'-二氯荧光素氧化为发荧光的2'，7'-二氯荧光素来测量过氧化物酶活性。

结果

1 MDa（0.5 mg / mL）或2 MDa（0.2 mg / mL）透明质酸与10 kDa（2.0 mg / mL）或100 kDa（0.1 mg / mL）透明质酸的混合物具有与人相似的粘度值全口水以剪切速率反映正常的口腔功能。与单一分子量的透明质酸相比，这些混合物的粘度值与人类整个唾液的粘度值更加相似。混合物抑制了羟基磷灰石表面的溶菌酶和过氧化物酶活性。但是，其抑制程度与仅1或2MDa的透明质酸没有什么不同。

结论

与单一分子量的透明质酸相比，透明质酸混合物的黏度值与人类整个唾液的黏度更相似，而对溶菌酶和过氧化物酶活性没有其他抑制作用。

临床相关性

透明质酸混合物为唾液替代品的开发提供了明显的优势。