当前位置: X-MOL 学术J. Funct. Foods › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans
Journal of Functional Foods ( IF 5.6 ) Pub Date : 2020-03-23 , DOI: 10.1016/j.jff.2020.103907
Haiqiang Chen , Shuang Wang , Aimei Zhou , Jianyin Miao , Jingyi Liu , Soottawat Benjakul

The residue rich in protein (up to 88.78%) obtained from round scad (Decapterus maruadsi) after oil extraction, was hydrolyzed by Alcalase to produce antioxidative peptides. The hydrolysate was separated by ultrafiltration, Sephadex G-15 and RP-HPLC, and the active fractions were screened by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline- 6 sulphonic acid) diammonium salt (ABTS), reducing power and oxygen radical absorbance capacity (ORAC). The obtained peptide with the highest antioxidative activity was then analyzed by HPLC and LC-MS/MS and its anti-aging effect was examined using Caenorhabditis elegans (C. elegans) model. The peptide (Ile-Leu-Gly-Ala-Thr-Ile-Asp-Asn-Ser-Lys, 1153.4 Da), identified by LC-MS/MS showed the highest antioxidant activity. C. elegans fed with the selected peptide had longer lifespan, higher survival rate, superoxide dismutase (SOD) and catalase (CAT) activities than the control group (P<0.05), demonstrating the promising anti-aging effects of the antioxidant peptide from defatted round scad.



中文翻译:

从脱脂圆鳞蛋白水解产物中纯化的新型抗氧化剂肽可延长秀丽隐杆线虫的寿命

提取油后从圆鳞Decapterus maruadsi)中获得的富含蛋白质的残渣(高达88.78%)被Alcalase水解以产生抗氧化肽。通过超滤,Sephadex G-15和RP-HPLC分离水解产物,并通过2,2-二苯基-1-picylhydrazyl(DPPH),2,2'-叠氮基双(3-乙基苯并噻唑啉-6)筛选活性级分磺酸)二铵盐(ABTS),降低功率和氧自由基吸收能力(ORAC)。然后通过HPLC和LC-MS / MS分析获得的具有最高抗氧化活性的肽,并使用秀丽隐杆线虫C. elegans)检查其抗衰老作用)模型。通过LC-MS / MS鉴定的肽(Ile-Leu-Gly-Ala-Thr-Ile-Asp-Asn-Ser-Lys,1153.4 Da)显示出最高的抗氧化活性。 饲喂所选肽的秀丽隐杆线虫比对照组具有更长的寿命,更高的存活率,超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性(P<0.05),证明了脱脂的抗氧化肽具有良好的抗衰老作用圆形

更新日期:2020-03-24
down
wechat
bug