In this study, the Jeju lava seawater (JLS) mineral-coated probiotics were developed and evaluated for their characteristics including intake stability and stimulation of intestinal immune system. Thirty percentage of JLS in culture medium provided the optimum viability and protein contents of probiotics. In in vitro continuous intake simulation model, minerals and salts stress in JLS elevated the tolerance of probiotics. Mineral-coated probiotics significantly increased the survival rate against the gastric juice (more than 20%) and bile salt broth (40%) compared with non-coated probiotics. Also, cell surface of mineral-coated probiotics showed more hydrophobicity and two times more affinity to chloroform. Cell surface hydrophobicity resulted in increased adhesive ability of mineral-coated probiotics. When the mineral-coated probiotics were treated on RAW 264.7 cells, the levels of IL-1β, an immune stimulatory cytokine, were increased twice more than that of red ginseng concentrate. In conclusion, the salt stress condition with JLS improved the intake stability and functions of probiotics. So, we expect that JLS mineral-coated probiotics could provide more significant health boosting effects than general probiotics.

在这项研究中，开发了济州熔岩海水（JLS）包被矿物的益生菌，并对其特性进行了评估，包括摄入稳定性和刺激肠道免疫系统。培养基中30％的JLS提供了最佳的益生菌活力和蛋白质含量。在体外连续摄入模拟模型，JLS中的矿物质和盐分胁迫提高了益生菌的耐受性。与未包衣的益生菌相比，矿物包被的益生菌显着提高了对胃液（超过20％）和胆汁盐汤（40％）的存活率。同样，矿物包裹的益生菌的细胞表面显示出更大的疏水性，并且对氯仿的亲和力是其两倍。细胞表面疏水性导致矿物包衣益生菌的粘附能力增强。当用矿物包裹的益生菌在RAW 264.7细胞上进行处理时，免疫刺激性细胞因子IL-1β的水平比红参浓缩物的水平增加了两倍。总之，JLS的盐胁迫条件改善了益生菌的摄入稳定性和功能。所以，