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Sensitive Multiplex Detection of MicroRNAs Based on Liquid Suspension Nano-Chip
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.aca.2020.03.026
Xiu Wang 1 , Wei Yuan 1 , Yuliang Xu 1 , Hang Yuan 1 , Fuyou Li 1
Affiliation  

Multiplex detection of microRNAs is highly desirable in trace analysis of clinical samples for early-stage disease diagnosis. In the present study, we report a new liquid suspension nano-chip technology for multiplex detection of microRNAs by detecting side scatter and fluorescence signals of molecular beacon-modified polystyrene nanoparticles, simultaneously. Polystyrene nanoparticles were encoded by distinguished intensity of scattered light with tunable sizes (120 nm, 200 nm and 280 nm). In the proposed detection system, the fluorescence of 5-carboxyfluorescein was recovered due to the structure change of molecular beacons from hairpin to rod-like shape caused by the hybridization of target microRNAs. The well-designed liquid suspension nano-chip system enabled quantitative detection of three colorectal cancer-related microRNAs with low limits of detection and high sensitivity. More importantly, for simulated clinical samples, three random mixed microRNAs were simultaneously detected by accurately decoding the scattering intensity of polystyrene nanoparticles of different sizes and quantitatively analyzed by fluorescence intensity. This technology provides a washing-free, sensitive, convenient and small loading volume strategy for detecting clinical multiplex nucleic acid samples.

中文翻译:

基于液体悬浮纳米芯片的MicroRNAs灵敏多重检测

microRNA 的多重检测在用于早期疾病诊断的临床样本的痕量分析中是非常理想的。在本研究中,我们报告了一种新的液体悬浮纳米芯片技术,通过同时检测分子信标修饰的聚苯乙烯纳米粒子的侧向散射和荧光信号来多重检测 microRNA。聚苯乙烯纳米颗粒由具有可调尺寸(120 nm、200 nm 和 280 nm)的散射光的显着强度编码。在所提出的检测系统中,由于靶 microRNA 的杂交导致分子信标的结构从发夹形变为杆状,从而恢复了 5-羧基荧光素的荧光。精心设计的液体悬浮纳米芯片系统能够以低检测限和高灵敏度对三种结直肠癌相关 microRNA 进行定量检测。更重要的是,对于模拟临床样本,通过准确解码不同尺寸聚苯乙烯纳米粒子的散射强度,同时检测三种随机混合的microRNA,并通过荧光强度进行定量分析。该技术为检测临床多重核酸样本提供了一种免洗、灵敏、方便、上样量小的策略。通过准确解码不同尺寸聚苯乙烯纳米粒子的散射强度,同时检测三种随机混合的microRNA,并通过荧光强度进行定量分析。该技术为检测临床多重核酸样本提供了一种免洗、灵敏、方便、上样量小的策略。通过准确解码不同尺寸聚苯乙烯纳米粒子的散射强度,同时检测三种随机混合的microRNA,并通过荧光强度进行定量分析。该技术为检测临床多重核酸样本提供了一种免洗、灵敏、方便、上样量小的策略。
更新日期:2020-05-01
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