Cell therapy is a promising strategy in which living cells or cellular materials are delivered to treat a variety of diseases. Here, we developed an electrospray bioprinting method to rapidly generate cell-laden hydrogel microspheres, which limit the migration of the captured cells and provide an immunologically privileged microenvironment for cell survival in vivo. Currently, therapeutic angiogenesis aims to induce collateral vessel formation after limb ischemia. However, the clinical application of gene and cell therapy has been impeded by concerns regarding its inefficacy, as well as the associated risk of immunogenicity and oncogenicity. In this study, hydrogel microspheres encapsulating VEGF-overexpressing HEK293T cells showed good safety via subcutaneously injecting into male C57BL/6 mice. In addition, these cell-modified microspheres effectively promoted angiogenesis in a mouse hind-limb ischemia model. Therefore, we demonstrated the great therapeutic potential of this approach to induce angiogenesis in limb ischemia, indicating that bioprinting has a bright future in cell therapy.

细胞疗法是一种有希望的策略，其中可以递送活细胞或细胞材料来治疗多种疾病。在这里，我们开发了一种电喷雾生物打印方法来快速生成充满细胞的水凝胶微球，从而限制捕获的细胞的迁移并为体内细胞存活提供免疫学上优越的微环境。目前，治疗性血管生成旨在诱导肢体缺血后侧支血管的形成。然而，基因和细胞疗法的临床应用由于其无效性以及免疫原性和致癌性的相关风险而受到阻碍。在这项研究中，包裹过表达VEGF的HEK293T细胞的水凝胶微球通过皮下注射给雄性C57BL / 6小鼠。此外，这些细胞修饰的微球在小鼠后肢缺血模型中有效促进了血管生成。因此，我们证明了这种方法在肢体缺血中诱导血管生成的巨大治疗潜力，表明生物打印在细胞治疗中具有光明的前景。