NAC [No apical meristem (NAM), Arabidopsis transcription activation factor (ATAF), Cup-shaped cotyledon (CUC)] transcription factors (TFs) play an important role in plant growth and response to various environmental stress. Drought stress is the primary factor limiting the growth and fruit quality of grapevines worldwide. However, the biological function of the NAC family members in grapevine is not clear. In this study, we reported that VvNAC08, a novel NAC transcription factor gene, was expressed after drought, salicylic acid (SA) and abscisic acid (ABA), jasmonic acid (JA) and melatonin (MT) treatments in grapevine. VvNAC08 was expressed in various tissues. The open reading frame (ORF) of VvNAC08 was 792 bp, encoding 263 amino acids. The VvNAC08 protein could bind to NACRS [CGTA/CACG] in yeast. When subjected to drought and dehydration stress, VvNAC08-overexpression (OE) Arabidopsis had a higher survival rate and a lower water loss rate than wild type (WT) plants. Under drought conditions, transgenic Arabidopsis overexpressing VvNAC08 had a lower malondialdehyde (MDA), H2O2 contents, but a higher peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) activities as well as more proline content. Moreover, the expressions of marker genes, including ABI5, AREB1, COR15A, COR47, P5CS, RD22, and RD29A, were up-regulated in VvNAC08-overexpression lines when subjected to drought treatments. The results suggest that the transgenic Arabidopsis overexpressing VvNAC08 enhances resistance to drought while up-regulating the expressions of ABA- and stress-related genes.

中文翻译：

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葡萄VvNAC08的过表达增强了转基因拟南芥的耐旱性。

NAC [无顶端分生组织（NAM），拟南芥转录激活因子（ATAF），杯状子叶（CUC）]转录因子（TFs）在植物生长和对各种环境胁迫的响应中起重要作用。干旱是限制全球葡萄树生长和果实品质的主要因素。但是，NAC家族成员在葡萄中的生物学功能尚不清楚。在这项研究中，我们报道了在干旱，水杨酸（SA）和脱落酸（ABA），茉莉酸（JA）和褪黑激素（MT）处理后的葡萄中表达新的NAC转录因子基因VvNAC08。VvNAC08在各种组织中表达。VvNAC08的开放阅读框（ORF）为792 bp，编码263个氨基酸。VvNAC08蛋白可以与酵母中的NACRS [CGTA / CACG]结合。当遭受干旱和脱水压力时，与野生型（WT）植物相比，VvNAC08-过表达（OE）拟南芥具有更高的存活率和更低的失水率。在干旱条件下，过表达VvNAC08的转基因拟南芥具有较低的丙二醛（MDA），H2O2含量，但具有较高的过氧化物酶（POD），超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及脯氨酸含量更高。此外，当经受干旱处理时，VvNAC08-过表达株系中包括ABI5，AREB1，COR15A，COR47，P5CS，RD22和RD29A在内的标记基因的表达上调。结果表明，过表达VvNAC08的转基因拟南芥增强了抗旱性，同时上调了ABA和胁迫相关基因的表达。在干旱条件下，过表达VvNAC08的转基因拟南芥具有较低的丙二醛（MDA），H2O2含量，但具有较高的过氧化物酶（POD），超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及脯氨酸含量更高。此外，当经受干旱处理时，VvNAC08-过表达株系中包括ABI5，AREB1，COR15A，COR47，P5CS，RD22和RD29A在内的标记基因的表达上调。结果表明，过表达VvNAC08的转基因拟南芥增强了抗旱性，同时上调了ABA和胁迫相关基因的表达。在干旱条件下，过表达VvNAC08的转基因拟南芥具有较低的丙二醛（MDA），H2O2含量，但具有较高的过氧化物酶（POD），超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及脯氨酸含量更高。此外，当经受干旱处理时，VvNAC08-过表达株系中包括ABI5，AREB1，COR15A，COR47，P5CS，RD22和RD29A在内的标记基因的表达上调。结果表明，过表达VvNAC08的转基因拟南芥增强了抗旱性，同时上调了ABA和胁迫相关基因的表达。干旱处理后，VvNAC08过表达株系中包括ABI5，AREB1，COR15A，COR47，P5CS，RD22和RD29A在内的标记基因的表达上调。结果表明，过表达VvNAC08的转基因拟南芥增强了抗旱性，同时上调了ABA和胁迫相关基因的表达。干旱处理后，VvNAC08过表达株系中包括ABI5，AREB1，COR15A，COR47，P5CS，RD22和RD29A在内的标记基因的表达上调。结果表明，过表达VvNAC08的转基因拟南芥增强了抗旱性，同时上调了ABA和胁迫相关基因的表达。