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Overexpression of HcSCL13, a Halostachys caspica GRAS transcription factor, enhances plant growth and salt stress tolerance in transgenic Arabidopsis.
Plant Physiology and Biochemistry ( IF 6.5 ) Pub Date : 2020-03-23 , DOI: 10.1016/j.plaphy.2020.03.020
Sheng Zhang 1 , Xuewen Li 1 , Shoude Fan 1 , Lianjie Zhou 1 , Yan Wang 1
Affiliation  

Salt is a major abiotic stress that negatively impacts plant growth and development. Research on the mechanisms of plant salt tolerance and the breeding of salt-tolerant plants is becoming an important research field. Transcription factors are master regulators that control the expression of many target genes, helping to regulate the response of plants to adverse conditions. GRAS are plant-specific transcription factors that play various roles in plant development and stress responses. However, the function of a GRAS gene identified in Halostachys caspica, a salt-tolerant plant with important ecological value, has not been determined. In this study, we characterized a novel gene (HcSCL13) encoding a GRAS transcription factor from H. caspica. Quantitative real-time (qRT)-PCR results indicated that HcSCL13 expression was induced by salt, drought and application of stress-related phytohormone abscisic acid (ABA). The HcSCL13 protein was localized in the nucleus with transactivation activity at the N terminus. Heterologous overexpression of HcSCL13 enhanced plant growth and salt tolerance in transgenic Arabidopsis. With HcSCL13 overexpression, plants had enhanced growth, as well as greater chlorophyll content, fresh weight and root elongation compared to the wild type. Transcriptomic analysis revealed that HcSCL13 overexpression affected the response to light/abiotic stimulus/hormone/organic substance, plant hormone signal-related and plant growth and development genes under normal and saline stress conditions. Taken together, these results indicate that HcSCL13 genes can modulate salt stress tolerance in Arabidopsis through the regulation of plant growth and the activation of gene expression.

中文翻译:

HcSCL13,Halostochys caspica GRAS转录因子的过表达,可增强转基因拟南芥中的植物生长和盐胁迫耐受性。

盐是一种主要的非生物胁迫,会对植物的生长和发育产生负面影响。对植物耐盐机理和耐盐植物育种的研究正成为重要的研究领域。转录因子是控制许多靶基因表达的主要调控因子,有助于调节植物对不利条件的反应。GRAS是植物​​特有的转录因子,在植物发育和胁迫响应中发挥多种作用。然而,尚未确定在具有重要生态价值的耐盐植物Halostochys caspica中鉴定出的GRAS基因的功能。在这项研究中,我们表征了一个新基因(HcSCL13),编码来自H. caspica的GRAS转录因子。实时定量(qRT)-PCR结果表明,盐诱导HcSCL13表达,干旱和胁迫相关植物激素脱落酸(ABA)的应用。HcSCL13蛋白位于N末端具有反式激活活性的核中。HcSCL13的异源过表达增强了转基因拟南芥中的植物生长和盐耐性。与野生型相比,HcSCL13过表达使植物具有增强的生长,以及更高的叶绿素含量,鲜重和根伸长。转录组学分析表明,在正常和盐胁迫条件下,HcSCL13的过表达影响了对光/非生物刺激物/激素/有机物,植物激素信号相关以及植物生长发育基因的反应。在一起
更新日期:2020-03-24
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