Abstract We present a laboratory experiment, developed around a pair of instrumental analysis techniques, Raman spectroscopy and ambient ionization mass spectrometry (MS), conducted by senior chemistry undergraduate students for bulk analysis of over-the-counter drugs using benchtop versions of the two instruments, as well as trace analysis of illicit drugs utilizing the corresponding portable instruments. The identification and confirmation of seized drugs is a routine operation in forensic laboratories that can be simplified using these two instrumental techniques in tandem. Bulk samples are readily analyzed using conventional Raman spectroscopy followed by paper cone spray ionization (PCSI) MS, while trace analysis is allowed by a simple single-substrate dual-analyzer method using surface enhanced Raman spectroscopy (SERS) from paper followed by paper spray ionization (PSI) MS. The introduction of these novel strategies to the students exposed them to potential career paths in forensic science by means of a hands-on experience that provided fundamental knowledge about this set of analytical methods, including intercomparisons of their performance, as well as practical issues like costs, regulations and databases. The use of portable instrumentation represents an additional learning experience for the students, considering that it is cutting-edge technology that is just now being transferred from academic to industrial labs and that it allows a valuable discussion of the advantages and disadvantages of bringing the laboratory to the field.

中文翻译：

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拉曼光谱与环境电离质谱联用：对快速简单的双仪器分析技术的法医实验室调查

摘要 我们介绍了一项实验室实验，该实验围绕拉曼光谱和环境电离质谱 (MS) 这两个仪器分析技术开发，由化学系本科生进行，使用这两种仪器的台式版本对非处方药进行批量分析。 ，以及使用相应的便携式仪器对非法药物进行痕量分析。缉获毒品的鉴定和确认是法医实验室的常规操作，可以同时使用这两种仪器技术来简化。使用传统的拉曼光谱，然后使用纸锥喷雾电离 (PCSI) MS，可以轻松分析散装样品，而痕量分析可以通过简单的单底物双分析仪方法使用纸的表面增强拉曼光谱 (SERS) 和纸喷雾电离 (PSI) MS 进行。向学生介绍这些新颖的策略，通过实践经验让他们接触法医学的潜在职业道路，这些经验提供了关于这套分析方法的基本知识，包括他们的表现的相互比较，以及成本等实际问题、法规和数据库。便携式仪器的使用代表了学生的额外学习体验，