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PP2A phosphatase is required for dendrite pruning via actin regulation in Drosophila.
EMBO Reports ( IF 7.7 ) Pub Date : 2020-03-24 , DOI: 10.15252/embr.201948870
Neele Wolterhoff 1 , Ulrike Gigengack 1 , Sebastian Rumpf 1
Affiliation  

Large-scale pruning, the developmentally regulated degeneration of axons or dendrites, is an important specificity mechanism during neuronal circuit formation. The peripheral sensory class IV dendritic arborization (c4da) neurons of Drosophila larvae specifically prune their dendrites at the onset of metamorphosis in an ecdysone-dependent manner. Dendrite pruning requires local cytoskeleton remodeling, and the actin-severing enzyme Mical is an important ecdysone target. In a screen for pruning factors, we identified the protein phosphatase 2 A (PP2A). PP2A interacts genetically with the actin-severing enzymes Mical and cofilin as well as other actin regulators during pruning. Moreover, Drosophila cofilin undergoes a change in localization at the onset of metamorphosis indicative of a change in actin dynamics. This change is abolished both upon loss of Mical and PP2A. We conclude that PP2A regulates actin dynamics during dendrite pruning.

中文翻译:

通过果蝇中的肌动蛋白调节,枝晶修剪需要PP2A磷酸酶。

大规模修剪,即轴突或树突的发育受调控的变性,是神经元回路形成过程中的重要特异性机制。果蝇幼虫的外周IV类树突状树突状神经(c4da)神经元在蜕变开始时以蜕皮激素依赖性的方式专门修剪其树突。树突修剪需​​要局部细胞骨架重塑,而切断肌动蛋白的酶Mical是蜕皮激素的重要靶标。在筛选修剪因子的过程中,我们鉴定了蛋白质磷酸酶2 A(PP2A)。PP2A在修剪过程中与肌动蛋白切断酶Mical和cofilin以及其他肌动蛋白调节剂发生遗传相互作用。此外,果蝇cofilin在变态开始时发生局部变化,这表明肌动蛋白动态变化。失去Mical和PP2A后,此更改将被废止。我们得出结论,PP2A在枝晶修剪过程中调节肌动蛋白的动力学。
更新日期:2020-03-24
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