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Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance.
BMC Plant Biology ( IF 5.3 ) Pub Date : 2020-03-23 , DOI: 10.1186/s12870-020-2329-2
Shi-Chao Sun 1 , Xian-Peng Xiong 1 , Xiao-Li Zhang 1 , Hong-Jie Feng 2 , Qian-Hao Zhu 3 , Jie Sun 1 , Yan-Jun Li 1
Affiliation  

BACKGROUND The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses has been studied in plants, however, limited is known about the 4CL genes in cotton (G. hirsutum L.) and their roles in response to drought stress. RESULTS We performed genome-wide identification of the 4CL genes in G. hirsutum and investigated the expression profiles of the identified genes in various cotton tissues and in response to stress conditions with an aim to identify 4CL gene(s) associated with drought tolerance. We identified 34 putative 4CL genes in G. hirsutum that were clustered into three classes. Genes of the same class usually share a similar gene structure and motif composition. Many cis-elements related to stress and phytohormone responses were found in the promoters of the Gh4CL genes. Of the 34 Gh4CL genes, 26 were induced by at least one abiotic stress and 10 (including Gh4CL7) were up-regulated under the polyethylene glycol (PEG) simulated drought stress conditions. Virus-induced gene silencing (VIGS) in cotton and overexpression (OE) in Arabidopsis thaliana were applied to investigate the biological function of Gh4CL7 in drought tolerance. The Gh4CL7-silencing cotton plants showed more sensitive to drought stress, probably due to decreased relative water content (RWC), chlorophyll content and antioxidative enzyme activity, increased stomatal aperture, and the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2). Arabidopsis lines overexpressing Gh4CL7, however, were more tolerant to drought treatment, which was associated with improved antioxidative enzyme activity, reduced accumulation of MDA and H2O2 and up-regulated stress-related genes under the drought stress conditions. In addition, compared to their respective controls, the Gh4CL7-silencing cotton plants and the Gh4CL7-overexpressing Arabidopsis lines had a ~ 20% reduction and a ~ 10% increase in lignin content, respectively. The expression levels of genes related to lignin biosynthesis, including PAL, CCoAOMT, COMT, CCR and CAD, were lower in Gh4CL7-silencing plants than in controls. Taken together, these results demonstrated that Gh4CL7 could positively respond to drought stress and therefore might be a candidate gene for improvement of drought tolerance in cotton. CONCLUSION We characterized the 4CL gene family in upland cotton and revealed a role of Gh4CL7 in lignin biosynthesis and drought tolerance.

中文翻译:

Gh4CL基因家族的表征揭示了Gh4CL7在干旱耐受性中的作用。

背景技术已经在植物中研究了4-香豆酸酯-CoA连接酶(4CL)在非生物胁迫下的功能,但是,关于棉花(G.hirsutum L.)中的4CL基因及其在干旱胁迫中的作用的知之甚少。结果我们对陆地棉中的4CL基因进行了全基因组鉴定,并研究了鉴定的基因在各种棉花组织中的表达谱,并响应了胁迫条件,目的是鉴定与耐旱性相关的4CL基因。我们在G. hirsutum中鉴定了34个假定的4CL基因,它们被分为三类。相同类别的基因通常共享相似的基因结构和基序组成。在Gh4CL基因的启动子中发现了许多与胁迫和植物激素反应有关的顺式元素。在34个Gh4CL基因中,在聚乙二醇(PEG)模拟的干旱胁迫条件下,至少一种非生物胁迫诱导了26种,其中10种(包括Gh4CL7)被上调。利用棉花的病毒诱导基因沉默(VIGS)和拟南芥中的过表达(OE)研究Gh4CL7在干旱耐受性中的生物学功能。Gh4CL7沉默的棉花植株对干旱胁迫表现出更高的敏感性,这可能是由于相对含水量(RWC),叶绿素含量和抗氧化酶活性降低,气孔孔径增加以及丙二醛(MDA)和过氧化氢(H2O2)含量所致。但是,过表达Gh4CL7的拟南芥品系对干旱处理的耐受性更高,这与抗氧化酶活性的提高有关,减少了干旱胁迫条件下MDA和H2O2的积累,并上调了胁迫相关基因。此外,与它们各自的对照相比,沉默Gh4CL7的棉花植物和过表达Gh4CL7的拟南芥品系的木质素含量分别降低了约20%和约10%。Gh4CL7沉默植物中与木质素生物合成相关的基因(包括PAL,CCoAOMT,COMT,CCR和CAD)的表达水平低于对照。综上所述,这些结果表明,Gh4CL7可以对干旱胁迫产生正响应,因此可能是提高棉花抗旱性的候选基因。结论我们表征了陆地棉上的4CL基因家族,并揭示了Gh4CL7在木质素生物合成和抗旱性中的作用。此外,与它们各自的对照相比,沉默Gh4CL7的棉花植物和过表达Gh4CL7的拟南芥品系的木质素含量分别降低了约20%和约10%。Gh4CL7沉默植物中与木质素生物合成相关的基因(包括PAL,CCoAOMT,COMT,CCR和CAD)的表达水平低于对照。综上所述,这些结果表明,Gh4CL7可以对干旱胁迫产生正响应,因此可能是提高棉花抗旱性的候选基因。结论我们表征了陆地棉上的4CL基因家族,并揭示了Gh4CL7在木质素生物合成和抗旱性中的作用。此外,与它们各自的对照相比,沉默Gh4CL7的棉花植物和过表达Gh4CL7的拟南芥品系的木质素含量分别降低了约20%和约10%。Gh4CL7沉默植物中与木质素生物合成相关的基因(包括PAL,CCoAOMT,COMT,CCR和CAD)的表达水平低于对照。综上所述,这些结果表明,Gh4CL7可以对干旱胁迫产生正响应,因此可能是提高棉花抗旱性的候选基因。结论我们表征了陆地棉上的4CL基因家族,并揭示了Gh4CL7在木质素生物合成和抗旱性中的作用。沉默Gh4CL7的棉花植物和过表达Gh4CL7的拟南芥品系的木质素含量分别降低了约20%和约10%。Gh4CL7沉默植物中与木质素生物合成相关的基因(包括PAL,CCoAOMT,COMT,CCR和CAD)的表达水平低于对照。综上所述,这些结果表明,Gh4CL7可以对干旱胁迫产生正响应,因此可能是提高棉花抗旱性的候选基因。结论我们表征了陆地棉上的4CL基因家族,并揭示了Gh4CL7在木质素生物合成和抗旱性中的作用。沉默Gh4CL7的棉花植物和过表达Gh4CL7的拟南芥品系的木质素含量分别降低了约20%和约10%。Gh4CL7沉默植物中与木质素生物合成相关的基因(包括PAL,CCoAOMT,COMT,CCR和CAD)的表达水平低于对照。综上所述,这些结果表明,Gh4CL7可以对干旱胁迫产生正响应,因此可能是提高棉花抗旱性的候选基因。结论我们表征了陆地棉上的4CL基因家族,并揭示了Gh4CL7在木质素生物合成和抗旱性中的作用。综上所述,这些结果表明,Gh4CL7可以对干旱胁迫产生积极响应,因此可能是提高棉花抗旱性的候选基因。结论我们表征了陆地棉上的4CL基因家族,并揭示了Gh4CL7在木质素生物合成和抗旱性中的作用。综上所述,这些结果表明,Gh4CL7可以对干旱胁迫产生积极响应,因此可能是提高棉花抗旱性的候选基因。结论我们表征了陆地棉上的4CL基因家族,并揭示了Gh4CL7在木质素生物合成和抗旱性中的作用。
更新日期:2020-04-22
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