Supplemental Digital Content is available in the text. Maternal-fetal crosstalk has been implicated in long-term control of the health of offspring, including transgenerational hypertension. However, current knowledge is limited regarding maternal influences on the gut and its microbiome in blood pressure control in offspring. Therefore, the current study was designed to test the hypothesis that maternal factors influence the gut-brain axis impacting hypertension in offspring. We elected to use captopril, an antihypertensive angiotensin-converting enzyme inhibitor that possesses antibacterial properties, for the study. Pregnant female spontaneously hypertensive rats and normotensive Wistar Kyoto rats were treated with captopril water (100 mg/[kg·day]) or sterile water throughout pregnancy and lactation. At weaning, the pups from dams drinking sterile water were continued with sterile water until 12 weeks of age. The male pups from dams drinking captopril water were divided at weaning into 2 groups: offspring drinking captopril water and offspring withdrawn from captopril water, then drinking sterile water until 12 weeks of age. Captopril changed gut microbiota of spontaneously hypertensive rat dams, and some of these changes were reflected in their 12-week-old male offspring. These 12-week-old spontaneously hypertensive rat male offspring exposed to captopril via dams demonstrated persistently decreased systolic blood pressure, decreased number of activated microglia and neuroinflammation, as well as improvement of gut inflammation and permeability. Therefore, maternal captopril treatment improves the dysregulated gut-brain axis in spontaneously hypertensive rat male offspring, providing conceptual support that targeting the gut-brain axis via the mother may be a viable strategy for control of hypertension in the offspring.

中文翻译：

---

母体使用卡托普利治疗持续改变肠-脑交流并减轻雄性后代的高血压

补充数字内容在文本中可用。母胎串扰与后代健康的长期控制有关，包括跨代高血压。然而，目前关于母体对肠道及其微生物组对后代血压控制的影响的知识有限。因此，本研究旨在检验母体因素影响影响后代高血压的肠脑轴的假设。我们选择使用卡托普利，一种具有抗菌特性的抗高血压血管紧张素转换酶抑制剂，用于研究。妊娠雌性自发性高血压大鼠和血压正常的 Wistar Kyoto 大鼠在整个孕期和哺乳期用卡托普利水 (100 mg/[kg·day]) 或无菌水治疗。断奶时，来自水坝的幼崽饮用无菌水继续使用无菌水直到 12 周龄。断奶时将饮用卡托普利水的母猪雄性幼仔分为2组：饮用卡托普利水的后代和退出卡托普利水的后代，然后饮用无菌水至12周龄。卡托普利改变了自发性高血压大鼠的肠道微生物群，其中一些变化反映在它们 12 周大的雄性后代中。这些 12 周大的自发性高血压大鼠雄性后代通过母体暴露于卡托普利，表现出持续降低的收缩压、活化的小胶质细胞和神经炎症的数量减少，以及肠道炎症和通透性的改善。所以，