Incidental soil ingestion is considered to be an important route of exposure to hydrophobic organic contaminants (HOCs), such as dichlorodiphenyl-trichloroethane (DDT). Contaminant ingestion often occurs during food consumption; however, knowledge on the influence of food on DDT bioavailability remains limited. In this study, the relative bioavailability (RBA) of soil DDTr (i.e., DDT and metabolites) was determined using an in vivo mouse model in the presence of eight kinds of food including rice, egg, pork, pear, soybean, bread, spinach, and milk powder. The values of DDTr-RBA ranged from 19.8 ± 10.9 to 114 ± 25.1%. DDTr-RBA was positively correlated with fat (r = 0.71) and negatively correlated with fiber (r = 0.63) content in food. A mechanistic study showed that fat enhanced micellarization and promoted the formation of chylomicron, which facilitated the dissolution and transport of DDTr in the intestinal tract. Bioaccessibility of DDTr was determined using a physiologically based in vitro method. The addition of lipase significantly improved the ability of the method to predict DDTr-RBA, indicating that the "fasted state" in vitro method required optimization for food scenarios. To the best of our knowledge, this is the first study to explore the mechanistic influence of food on DDTr-RBA and provide important knowledge on dietary approaches for reducing exposure to HOCs.

中文翻译：

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食物对土壤中滴滴涕及其代谢产物体内生物利用度的影响。

偶然的土壤摄入被认为是暴露于疏水有机污染物（HOC）（如二氯二苯基三氯乙烷（DDT））的重要途径。食物摄入过程中经常发生污染物的摄入。但是，有关食物对滴滴涕生物利用度影响的知识仍然有限。在这项研究中，使用体内小鼠模型在八种食物（包括大米，鸡蛋，猪肉，梨，大豆，面包，菠菜）存在下，测定了土壤DDTr（即DDT和代谢产物）的相对生物利用度（RBA）和奶粉。DDTr-RBA的值介于19.8±10.9至114±25.1％之间。DDTr-RBA与食物中的脂肪呈正相关（r = 0.71），与纤维含量呈负相关（r = 0.63）。一项机理研究表明，脂肪会增强胶束化并促进乳糜微粒的形成，这有助于DDTr在肠道中的溶解和运输。使用基于生理的体外方法确定DDTr的生物可及性。脂肪酶的加入显着提高了该方法预测DDTr-RBA的能力，表明“禁食状态”体外方法需要针对食品场景进行优化。据我们所知，这是第一个研究食物对DDTr-RBA的机械影响并提供有关饮食方法以减少HOC暴露的重要知识的研究。体外方法需要针对食品场景进行优化。据我们所知，这是第一个研究食物对DDTr-RBA的机械影响并提供有关饮食方法以减少HOC暴露的重要知识的研究。体外方法需要针对食品场景进行优化。据我们所知，这是第一个研究食物对DDTr-RBA的机械影响并提供有关饮食方法以减少HOC暴露的重要知识的研究。