Nanochannel-based analytical techniques have great potential applications for nucleic acid sequencing and high sensitivity detection of biological molecules. However, the sensitivity of conventional solid-state nanochannel sensors is hampered by a lack of effective signal amplification strategies, which has limited its utility in the field of analytical chemistry. Here we selected a solid-state nanochannnel modified with polyethylenimine and Zr4+ in combination with graphene oxide as the sensing platform. The high-performance sensor is based upon the change of the surface charge of the nanochannel, which is resulted from DNA cascade signal amplification in solution. The target miRNA (miR-122) can be indirectly quantitated with a detection limit of 97.2 aM with an excellent selectivity. Depending on the nucleic acid's hybridization and configuration transform, the designed nanochannel sensing systems can realize the intelligent detection of multiple liver cancer-related miRNA (miR-122 and miR Let-7a) integrating with cascaded INHIBIT-OR logic gate to provide theoretical guidance and technical support for clinical diagnosis and therapeutic evaluation of liver cancer.

中文翻译：

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仿生纳米通道逻辑平台的制造及其在与肝癌相关的miRNA的智能检测中的应用。

基于纳米通道的分析技术在核酸测序和生物分子的高灵敏度检测方面具有巨大的潜在应用。然而，由于缺乏有效的信号放大策略，传统的固态纳米通道传感器的灵敏度受到了限制，这限制了其在分析化学领域的实用性。在这里，我们选择了用聚乙烯亚胺和Zr4 +结合氧化石墨烯修饰的固态纳米通道作为传感平台。高性能传感器基于纳米通道表面电荷的变化，这是由溶液中的DNA级联信号放大引起的。靶miRNA（miR-122）可以以97.2 aM的检测限进行间接定量，并具有出色的选择性。取决于核酸'