The rapid proliferation of new synthetic cannabinoid receptor agonists (SCRAs) has initiated considerable interest in the development of so-called "untargeted" screening strategies. One of these new screening technologies involves the activity-based detection of SCRAs. In this study, we evaluated whether (synthetic) cannabinoid activity can be detected in oral fluid (OF) and, if so, whether it correlates with SCRA concentrations. OF was collected at several time points in a placebo-controlled JWH-018 administration study. The outcome of the cell-based cannabinoid reporter system, which monitored the cannabinoid receptor activation, was compared to the quantitative data for JWH-018, obtained via a validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. A total of 175 OF samples were collected and analyzed via both methods. The cannabinoid reporter assay correctly classified the vast majority of the samples as either negative (<0.25 ng/mL; 74/75 = 99%) or having low (0.25-1.5 ng/mL; 16/16 = 100% and 1.5-10 ng/mL; 37/41 = 90%), mid (10-100 ng/mL; 23/25 = 92%) or high (>100 ng/mL; 16/18 = 89%) JWH-018 concentrations. Passing-Bablok regression analysis yielded a good linear correlation, with no proportional difference between both methods (slope 0.97; 95% confidence interval 0.86-1.14) and only a small systematic difference. This is the first study to demonstrate the applicability of an untargeted, activity-based approach for SCRA detection in OF. Additionally, the outcome of the cannabinoid reporter assay was compared to the gold standard (LC-MS/MS), showing a good correlation between both methods, indicating that the cannabinoid reporter assay can be used for an estimation of drug concentrations.

中文翻译：

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雾化后口服液中基于合成大麻素受体激动剂JWH-018的基于半定量活动的检测。

新的合成大麻素受体激动剂（SCRA）的迅速扩散引起了人们对所谓“非靶向”筛选策略发展的极大兴趣。这些新的筛选技术之一涉及基于活动的SCRA检测。在这项研究中，我们评估了是否可以在口腔液（OF）中检测到（合成的）大麻素活性，如果可以，还可以检测到其是否与SCRA浓度相关。在安慰剂对照的JWH-018管理研究中的几个时间点收集了OF。将监测大麻素受体活化的基于细胞的大麻素报告系统的结果与通过验证的液相色谱-串联质谱法（LC-MS / MS）获得的JWH-018定量数据进行了比较。通过这两种方法收集并分析了总共175个OF样品。大麻素报告基因分析将绝大多数样品正确分类为阴性（<0.25 ng / mL; 74/75 = 99％）或低（0.25-1.5 ng / mL; 16/16 = 100％和1.5-10） ng / mL； 37/41 = 90％），中等（10-100 ng / mL； 23/25 = 92％）或高浓度（> 100 ng / mL； 16/18 = 89％）的JWH-018浓度。Passing-Bablok回归分析产生了良好的线性相关性，两种方法之间没有比例差异（斜率0.97； 95％置信区间0.86-1.14），并且只有很小的系统差异。这是第一个证明非目标，基于活动的方法在OF中进行SCRA检测的适用性的研究。此外，将大麻素报告基因检测的结果与金标准品（LC-MS / MS）进行了比较，