Human health is recently affected by several factors in which food contamination is one of the most dangerous elements that damage directly on our bodies. In this study, we provided a novel approach for the rapid detection of Salmonella sp. at the molecular level using the response of Saccharomyces cerevisiae’s vacuoles. First, an augmentation of vacuoles intensity was observed by confocal microscopy after treating Salmonella strains with yeast cells. Second, the vacuolar enzymes were isolated and then analyzed by two‐dimensional electrophoresis for the screening of specific biomarkers. After that, various recombinant yeasts containing exclusive biomarkers were constructed by fusing these biomarkers with several fluorescent proteins. Finally, the recombinant strains showed the ability to detect Salmonella strains specifically by appropriate fluorescent signals from 20 CFU/mL after 15 Min of exposure.

中文翻译：

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酵母液泡蛋白的反应：沙门氏菌新型快速工具。筛选

最近，人类健康受到多种因素的影响，其中食物污染是直接损害人体的最危险因素之一。在这项研究中，我们提供了一种快速检测沙门氏菌sp。的新颖方法。在分子水平上利用酿酒酵母液泡的反应 首先，在治疗沙门氏菌后，通过共聚焦显微镜观察到液泡强度增加酵母细胞株。其次，分离液泡酶，然后通过二维电泳进行分析，以筛选特定的生物标志物。之后，通过将这些生物标志物与几种荧光蛋白融合，构建了各种包含独家生物标志物的重组酵母。最后，重组菌株显示出能够通过暴露15分钟后通过来自20 CFU / mL的适当荧光信号特异性检测沙门氏菌菌株的能力。