Abstract

Surface charge of the skin matrix is an extremely important parameter in leather making process because effectivity of the chemicals largely depends on the surface charge of the skin matrix. In the present research work, isoelectric point (Ip) and point of zero charge (pzc) are used to predict the distribution of electric potential and charge at the skin tissue–liquid interface after chemical and enzymatic unhairing of the skin. In this study, the determination of zeta potential and isoelectric point for relimed tissues processed by chemical and enzymatic methods was performed using a zeta potential analyzer and a titrimetric method. These results indicate that enzyme-treated tissues had lower isoelectric point than the chemical-treated tissues, which has been explained by studying the amino acid composition of the tissues. The tissue samples were also characterized by FTIR–ATR and solid-state ¹³C NMR spectroscopy to understand the chemical/structural changes occurred due to enzymatic treatment. The lower Ip/pzc of the tissue obtained by enzymatic process ensured maximum uptake of chemicals, making it an eco-benign process.

Graphic abstract

中文翻译：

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酶引起的皮肤变化及其影响

摘要

皮肤基质的表面电荷是皮革制造过程中极为重要的参数，因为化学物质的有效性在很大程度上取决于皮肤基质的表面电荷。在当前的研究工作中，等电点（Ip）和零电荷点（pzc）用于预测化学和酶促脱毛后皮肤组织-液体界面处的电势和电荷分布。在这项研究中，使用zeta电位分析仪和滴定法对通过化学和酶法处理过的组织的zeta电位和等电点进行测定。这些结果表明酶处理的组织具有比化学处理的组织低的等电点，这已经通过研究组织的氨基酸组成来解释。^用13 C NMR光谱了解由于酶处理引起的化学/结构变化。通过酶促过程获得的组织的较低的Ip / pzc确保最大程度地吸收化学物质，使其成为一种生态良性过程。

图形摘要