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Fabrication of aptamer modified TiO2 nanofibers for specific capture of circulating tumor cells.
Colloids and Surfaces B: Biointerfaces ( IF 5.8 ) Pub Date : 2020-03-21 , DOI: 10.1016/j.colsurfb.2020.110985 Hui Liu 1 , Na Sun 1 , Pi Ding 1 , Changchong Chen 1 , Zeen Wu 2 , Weipei Zhu 3 , Lifen Liu 3 , Zhili Wang 1 , Renjun Pei 1
Colloids and Surfaces B: Biointerfaces ( IF 5.8 ) Pub Date : 2020-03-21 , DOI: 10.1016/j.colsurfb.2020.110985 Hui Liu 1 , Na Sun 1 , Pi Ding 1 , Changchong Chen 1 , Zeen Wu 2 , Weipei Zhu 3 , Lifen Liu 3 , Zhili Wang 1 , Renjun Pei 1
Affiliation
Herein, we developed an inexpensive titanium dioxide (TiO2) nanofiber substrate for efficient and selective capture of circulating tumor cells (CTCs) from mimic patients' samples. The TiO2 nanofiber substrates were fabricated by electrospinning in combination with the calcination process. The surface of nanofiber substrates was modified with the anti-adhesion molecule, bovine serum albumin (BSA) and the nucleolin aptamer AS1411, wherein, aptamer AS1411 specifically binds to the nucleolin protein overexpressed on the membrane surface of cancer cells. The formed TiO2 nanofiber substrates exhibited high efficacy and specificity to capture nucleolin positive cells through synergistic topographic interactions. Using the rare number of cell capture experiments, the capture efficiency of up to 75 % was achieved on the surface of the nanofiber substrate for rare number target cells spiked in the white blood cells (WBCs) from 1 mL whole blood samples. In conclusion, this study highlighted the potential of the TiO2-BSA-biotin-AS1411 nanofiber substrate as a highly efficient platform to realize the selective and specific capture of rare CTCs in the clinical settings.
中文翻译:
适体修饰的TiO2纳米纤维的制备,用于特异性捕获循环肿瘤细胞。
本文中,我们开发了一种廉价的二氧化钛(TiO2)纳米纤维基材,用于从模拟患者的样品中高效,选择性地捕获循环肿瘤细胞(CTC)。TiO2纳米纤维基材是通过电纺丝结合煅烧工艺制备的。用抗粘附分子,牛血清白蛋白(BSA)和核仁适体AS1411修饰纳米纤维基质的表面,其中,适体AS1411特异性结合在癌细胞膜表面上过表达的核仁蛋白。形成的TiO2纳米纤维底物表现出高效率和特异性,可通过协同形貌相互作用捕获核仁蛋白阳性细胞。使用罕见的细胞捕获实验,对于从1毫升全血样品中掺入白细胞(WBC)的稀有靶细胞,在纳米纤维基质表面上的捕获效率高达75%。总之,这项研究强调了TiO2-BSA-生物素-AS1411纳米纤维基质作为高效平台的潜力,可在临床环境中实现对稀有CTC的选择性和特异性捕获。
更新日期:2020-03-22
中文翻译:
适体修饰的TiO2纳米纤维的制备,用于特异性捕获循环肿瘤细胞。
本文中,我们开发了一种廉价的二氧化钛(TiO2)纳米纤维基材,用于从模拟患者的样品中高效,选择性地捕获循环肿瘤细胞(CTC)。TiO2纳米纤维基材是通过电纺丝结合煅烧工艺制备的。用抗粘附分子,牛血清白蛋白(BSA)和核仁适体AS1411修饰纳米纤维基质的表面,其中,适体AS1411特异性结合在癌细胞膜表面上过表达的核仁蛋白。形成的TiO2纳米纤维底物表现出高效率和特异性,可通过协同形貌相互作用捕获核仁蛋白阳性细胞。使用罕见的细胞捕获实验,对于从1毫升全血样品中掺入白细胞(WBC)的稀有靶细胞,在纳米纤维基质表面上的捕获效率高达75%。总之,这项研究强调了TiO2-BSA-生物素-AS1411纳米纤维基质作为高效平台的潜力,可在临床环境中实现对稀有CTC的选择性和特异性捕获。