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The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.
iScience ( IF 5.8 ) Pub Date : 2020-03-21 , DOI: 10.1016/j.isci.2020.101004
Tirthankar Sinha 1 , Muna I Naash 1 , Muayyad R Al-Ubaidi 1
Affiliation  

The neural retina and retinal pigment epithelium (RPE) maintain a symbiotic metabolic relationship, disruption of which leads to debilitating vision loss. The current study was undertaken to identify the differences in the steady-state metabolite levels and the pathways functioning between bona fide neural retina and RPE. Global metabolomics and cluster analyses identified 650 metabolites differentially modulated between the murine neural retina and RPE. Of these, 387 and 163 were higher in the RPE and the neural retina, respectively. Further analysis coupled with transcript and protein level investigations revealed that under normal physiological conditions, the RPE utilizes the pentose phosphate (>3-fold in RPE), serine (>10-fold in RPE), and sphingomyelin biosynthesis (>5-fold in RPE) pathways. Conversely, the neural retina relied mostly on glycolysis. These results show how the RPE and the neural retina have acquired an efficient, complementary and metabolically diverse symbiotic niche to support each other's distinct functions.



中文翻译:

通过利用差异代谢途径支持神经视网膜和视网膜色素上皮之间的共生关系。

神经视网膜和视网膜色素上皮 (RPE) 保持共生代谢关系,其破坏导致视力丧失。目前的研究旨在确定稳态代谢物水平的差异以及真正的代谢物之间的功能途径神经视网膜和RPE。全球代谢组学和聚类分析确定了 650 种在小鼠神经视网膜和 RPE 之间差异调节的代谢物。其中,387 和 163 分别在 RPE 和神经视网膜中较高。进一步的分析与转录和蛋白质水平的研究相结合,表明在正常生理条件下,RPE 利用磷酸戊糖(在 RPE 中 > 3 倍)、丝氨酸(在 RPE 中 > 10 倍)和鞘磷脂生物合成(在 RPE 中 > 5 倍)。 RPE) 途径。相反,神经视网膜主要依赖糖酵解。这些结果表明 RPE 和神经视网膜如何获得有效、互补和代谢多样化的共生生态位,以支持彼此的不同功能。

更新日期:2020-03-21
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