With recent advances in nanotechnology, debranched starch nanoparticle (DBS-NP) materials have attracted considerable interest from the fields of functional food, biomedicine, and material science, thanks to their small size, biodegradability, biocompatibility, sustainability, and non-hazardous effects on health and the environment. In this study, DBS-NP was fabricated using an eco-friendly method involving ultrasonication combined with recrystallization. The effects of ultrasonication and recrystallization times on the morphology, particle size, and crystal structure of the DBS-NPs were systematically investigated. Compared with the DBS-NPs prepared using ultrasonication treatment only, the DBS-NPs formed using ultrasonication combined with recrystallization were uniform in size and well distributed in aqueous solution. Moreover, the maximum encapsulation efficiency and loading capacity of the epigallocatechin gallate (EGCG) in the DBS-NPs with ultrasonication treatment reached 88.35% and 22.75%, respectively. The particle sizes of the EGCG@DBS-NP were more stable at a neutral pH (7.4) than at an acidic pH (2.1). The EGCG in the EGCG@DBS-NP displayed excellent radical scavenging activity and antibacterial effects, and cell assays demonstrated that the EGCG@DBS-NP was non-toxic and highly biocompatible.

随着纳米技术的最新发展，脱支淀粉纳米颗粒（DBS-NP）材料因其体积小，可生物降解性，生物相容性，可持续性和对环境的无害影响，引起了功能食品，生物医学和材料科学领域的极大关注。健康与环境。在这项研究中，DBS-NP是使用环保方法制造的，该方法涉及超声处理和重结晶。系统地研究了超声和重结晶时间对DBS-NPs的形态，粒径和晶体结构的影响。与仅使用超声处理制备的DBS-NPs相比，通过超声处理与重结晶结合形成的DBS-NPs大小均匀且在水溶液中分布均匀。此外，超声处理后，表没食子儿茶素没食子酸酯（EGCG）在DBS-NPs中的最大包封率和载药量分别达到88.35％和22.75％。EGCG @ DBS-NP的粒径在中性pH（7.4）下比在酸性pH（2.1）下更稳定。EGCG @ DBS-NP中的EGCG表现出优异的自由基清除活性和抗菌作用，细胞分析表明EGCG @ DBS-NP无毒且具有高度生物相容性。