Peste des petits ruminants (PPR) is a severe, highly contagious and fatal viral disease of small ruminants causing high economic losses. Therefore, the disease was targeted for eradication by 2030. The aim of this study was to investigate for the first time the molecular and pathological characterization of the circulating PPR virus (PPRV) in sheep and goat in Palestine. Samples were collected from suspected necropsy cases of sheep and goats during recent outbreaks in two provinces in Palestine between 2017 and 2019. In this study, severe PPR outbreaks occurred in sheep and goats causing typical lesions which include erosive and ulcerative stomatitis, bronchointerstitial pneumonia and severe enteritis. For the molecular investigation of PPRV, suspected animals were examined for the presence of PPRV by RT‐PCR. PPRV genome was detected in all samples. Subsequently, two samples were used for N gene sequencing and phylogenetic analysis of PPRV isolates. The nucleotide sequence and phylogenetic analysis indicated that the Palestinian PPRV isolates were genetically clustered within the lineage IV isolates of the virus among populations of sheep and goats which most prevalent in Asia, the Middle East and recently Africa. Further analysis showed that the Palestinian isolates were closely related to those described in Turkey and Iraq, suggesting a common origin of PPRV isolates in the region. This information is critical to understand the molecular epidemiology of this disease in the region and helps to develop appropriate control measures for eradication of this disease.

中文翻译：

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2017-2019 年巴勒斯坦暴发小反刍兽疫病毒 (PPRV) 的分子和病​​理学研究

小反刍兽疫（PPR）是一种严重的、高度传染性和致命性的小型反刍动物病毒性疾病，造成巨大的经济损失。因此，该疾病的目标是到 2030 年根除。这项研究的目的是首次调查巴勒斯坦绵羊和山羊中循环的 PPR 病毒 (PPRV) 的分子和病​​理特征。样本是从 2017 年至 2019 年巴勒斯坦两个省份近期爆发的绵羊和山羊疑似尸检病例中采集的。在这项研究中，绵羊和山羊发生了严重的小反刍兽疫疫情，造成典型病变，包括糜烂性和溃疡性口腔炎、支气管间质性肺炎和严重的小反刍兽疫。肠炎。为了进行 PPRV 的分子研究，通过 RT-PCR 检查可疑动物是否存在 PPRV。所有样本中均检测到 PPRV 基因组。随后，两个样本用于 PPRV 分离株的 N 基因测序和系统发育分析。核苷酸序列和系统发育分析表明，巴勒斯坦 PPRV 分离株在基因上聚集在绵羊和山羊群体中病毒的 IV 谱系分离株内，该病毒在亚洲、中东和最近的非洲最为流行。进一步分析表明，巴勒斯坦分离株与土耳其和伊拉克描述的分离株密切相关，表明该地区 PPRV 分离株具有共同起源。这些信息对于了解该地区该疾病的分子流行病学至关重要，并有助于制定根除该疾病的适当控制措施。