Although human adipose‐derived stem cells (hASCs) have great potential as a cell source for stem cell therapy due to their capacity of self‐renewal, long‐term growth, and multipotency with high obtainability through lipoaspiration, little is known about the effects of micro/nano dual‐scale topography on the proliferation and differentiation of the hASCs. In this study, the effects of micro/nano dual‐scale crossed sinusoidal wavy patterns (SIMNs) on the hASC behaviors including cellular alignment, proliferation, and endothelial differentiation are investigated. Various types of microscale, nanoscale, and SIMNs are fabricated on a single polystyrene surface in a step‐gradient manner based on the combined fabrication processes of deep X‐ray lithography, polydimethylsiloxane surface wrinkling, and thermal nanoimprinting process. The cellular alignment, proliferation, and endothelial differentiation of the hASCs are promoted on the SIMNs compared to the microscale or nanoscale sinusoidal wavy patterns. A micro/nano dual‐scale pattern of SIMN_{160_500}, which has a micro‐scale sinusoidal wavy pattern with a wavelength of 160 µm crossed with a nanoscale sinusoidal wavy pattern with a wavelength of 500 nm, is found to be a most effective pattern for synergistically promoting both proliferation and endothelial differentiation of the hASCs with 1.2‐fold and 2‐fold increases, respectively.

中文翻译：

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微观/纳米双尺度正弦波状波形模式，以协同促进人类脂肪干细胞的增殖和内皮分化。

尽管人类脂肪干细胞（hASCs）具有自我更新，长期生长和通过吸脂获得高功效的多能性，作为干细胞治疗的细胞来源具有巨大潜力，但人们对它的作用知之甚少hASCs增殖和分化的微观/纳米双重尺度地形。在这项研究中，研究了微/纳米双尺度交叉正弦波形模式（SIMN）对hASC行为的影响，包括细胞排列，增殖和内皮分化。基于深X射线光刻，聚二甲基硅氧烷表面起皱和热纳米压印工艺的组合制造工艺，可以在单个聚苯乙烯表面上以逐步梯度的方式制造各种类型的微米级，纳米级和SIMN。细胞排列 与微尺度或纳米尺度的正弦波状波形相比，在SIMN上促进了hASC的增殖和内皮分化。SIMN的微米/纳米双尺度模式_{160_500}具有_160μm的微尺度正弦波状波状图案与500nm的纳米尺度正弦波状波状交叉，被认为是协同促进hASCs增殖和内皮分化的最有效模式分别增加1.2倍和2倍。