The purpose of current investigation was to explore the role of brassinosteroids (BRs) in Zea mays L . var. DKC 9106 seedlings subjected to salt stress. The seedlings were raised under controlled laboratory conditions and subjected to different concentrations of NaCl (0, 40, 60, 80, 100 mM) for 10 days. The impact of pre-sowing treatment of both 28-homobrassionolide (HBL) and 24-epibrassinolide (EBL) on defense system of Z. mays L. under salt stress was studied by analyzing Na + and K + ions, malondialdehyde content (MDA), antioxidative enzymes activities (peroxidase, POD; catalase, CAT; dehydroascorbate reductase, DHAR; monodehydroascorbate reductase, MDHAR), osmoprotectants (proline, glycine betaine, mannitol, and total osmolytes content), total phenolic content, total flavonoid content, and 1,1-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity. The results of our finding showed that treatment of both HBL and EBL under high salt stress balanced the ionic status by decreasing the Na + ions content by 21.23% and 38.94%, respectively, and enhancing the K + ions content by 51.94% and 26.66%, respectively. Treatment of both BRs also overcome the oxidative damage induced due to salinity stress by reducing the MDA accumulation 19.50% and 45.0%, respectively, and enhancing the activities of antioxidative enzymes. The osmoprotectants: proline (50.08% and 17.03%), glycine betaine (35.57% and 28.16%), and mannitol content (2.80% and 20.98%) were markedly increased by the treatment of both HBL and EBL, respectively. Further, treatment of both HBL and EBL also increased the total phenolic content by 11.68% and 5.80%, total flavonoid content by 31.56 and 31.09% and DPPH free radical scavenging activity by 37.99% and 77.41%, respectively. Overall the treatment of BRs before seed sowing considerably conquer the salinity-induced damage by stimulating functional components of antioxidative defense system and ultimately reduced oxidative damage.

中文翻译：

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油菜素类固醇调节盐胁迫玉米幼苗抗氧化防御系统的功能成分

当前调查的目的是探索油菜素类固醇 (BRs) 在玉米中的作用。变种 DKC 9106 幼苗遭受盐胁迫。在受控的实验室条件下培育幼苗，并在不同浓度的 NaCl（0、40、60、80、100 mM）下培养 10 天。通过分析Na + 和K + 离子、丙二醛含量（MDA），研究了28-高油菜内酯（HBL）和24-表油菜内酯（EBL）的播种前处理对盐胁迫下玉米防御系统的影响，抗氧化酶活性（过氧化物酶，POD；过氧化氢酶，CAT；脱氢抗坏血酸还原酶，DHAR；单脱氢抗坏血酸还原酶，MDHAR）、渗透保护剂（脯氨酸、甘氨酸甜菜碱、甘露醇和总渗透物含量）、总酚含量、总黄酮含量和 1，1-二苯基苦基肼 (DPPH) 自由基清除活性。我们的研究结果表明，在高盐胁迫下对 HBL 和 EBL 的处理通过分别将 Na + 离子含量降低 21.23% 和 38.94% 并将 K + 离子含量提高 51.94% 和 26.66% 来平衡离子状态， 分别。两种BRs的处理还通过将MDA积累分别降低19.50%和45.0%并增强抗氧化酶的活性来克服盐胁迫引起的氧化损伤。渗透保护剂：脯氨酸（50.08% 和 17.03%）、甘氨酸甜菜碱（35.57% 和 28.16%）和甘露醇含量（2.80% 和 20.98%）分别通过 HBL 和 EBL 的处理显着增加。此外，HBL 和 EBL 的处理也使总酚含量增加了 11.68% 和 5.80%，总黄酮含量分别为 31.56% 和 31.09%，DPPH 自由基清除活性分别为 37.99% 和 77.41%。总的来说，在播种前对 BR 进行处理，通过刺激抗氧化防御系统的功能成分并最终减少氧化损伤，大大克服了盐度引起的损伤。